Impact of Cd and Pb on the photosynthetic and antioxidant systems of Hemerocallis citrina Baroni as revealed by physiological and transcriptomic analyses.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES Plant Cell Reports Pub Date : 2024-09-04 DOI:10.1007/s00299-024-03312-w
Boxue Zhang, Zebin Li, Yuwei Feng, Sunara Qaharaduqin, Wei Liu, Yongqing Yan
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Abstract

Key message: Cd induces photosynthetic inhibition and oxidative stress damage in H. citrina, which mobilizes the antioxidant system and regulates the expression of corresponding genes to adapt to Cd and Pb stress. Cd and Pb are heavy metals that cause severe pollution and are highly hazardous to organisms. Physiological measurements and transcriptomic analysis were combined to investigate the effect of 5 mM Cd or Pb on Hemerocallis citrina Baroni. Cd significantly inhibited H. citrina growth, while Pb had a minimal impact. Both Cd and Pb suppressed the expression levels of key chlorophyll synthesis genes, resulting in decreased chlorophyll content. At the same time, Cd accelerated chlorophyll degradation. It reduced the maximum photochemical efficiency of photosystem (PS) II, damaging the oxygen-evolving complex and leading to thylakoid dissociation. In contrast, no such phenomena were observed under Pb stress. Cd also inhibited the Calvin cycle by down-regulating the expression of Rubisco and SBPase genes, ultimately disrupting the photosynthetic process. Cd impacted the light reaction processes by damaging the antenna proteins, PS II and PS I activities, and electron transfer rate, while the impact of Pb was weaker. Cd significantly increased reactive oxygen species and malondialdehyde accumulation, and inhibited the activities of antioxidant enzymes and the expression levels of the corresponding genes. However, H. citrina adapted to Pb stress by the recruitment of antioxidant enzymes and the up-regulation of their corresponding genes. In summary, Cd and Pb inhibited chlorophyll synthesis and hindered the light capture and electron transfer processes, with Cd exerting great toxicity than Pb. These results elucidate the physiological and molecular mechanisms by which H. citrina responds to Cd and Pb stress and provide a solid basis for the potential utilization of H. citrina in the greening of heavy metal-polluted lands.

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生理和转录组分析显示镉和铅对 Hemerocallis citrina Baroni 的光合作用和抗氧化系统的影响
关键信息:镉会诱导枸橘光合抑制和氧化应激损伤,从而调动抗氧化系统并调节相应基因的表达以适应镉和铅的胁迫。镉和铅是造成严重污染的重金属,对生物体危害极大。本研究结合生理学测量和转录组分析,探讨了 5 mM Cd 或 Pb 对 Hemerocallis citrina Baroni 的影响。镉明显抑制了Hemerocallis citrina的生长,而铅的影响很小。镉和铅都抑制了关键叶绿素合成基因的表达水平,导致叶绿素含量下降。同时,镉加速了叶绿素降解。它降低了光系统(PS)II 的最大光化学效率,破坏了氧气生成复合物,导致类木质解离。相比之下,在铅胁迫下没有观察到此类现象。镉还通过下调 Rubisco 和 SBPase 基因的表达来抑制卡尔文循环,最终破坏光合作用过程。镉通过破坏触角蛋白、PS II 和 PS I 活性以及电子传递速率来影响光反应过程,而铅的影响较弱。镉会明显增加活性氧和丙二醛的积累,抑制抗氧化酶的活性和相应基因的表达水平。然而,H. citrina通过招募抗氧化酶及其相应基因的上调来适应铅胁迫。总之,镉和铅抑制了叶绿素的合成,阻碍了光捕获和电子传递过程,其中镉的毒性大于铅。这些结果阐明了枸橘应对镉和铅胁迫的生理和分子机制,为枸橘在重金属污染土地绿化中的潜在利用提供了坚实的基础。
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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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