A novel non-invasive method for measuring the spatial kinematic behavior of cardiomyocytes regulated by mechanical cues

IF 10 2区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY Materials Today Physics Pub Date : 2024-09-01 DOI:10.1016/j.mtphys.2024.101543
Si Tang , Huiyao Shi , Jialin Shi , Zhenghua Wang , Dingyi Wang , Chanmin Quanmin Su , Lianqing Liu
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Abstract

The intact heart undergoes complex and multiscale mechanical remodeling processes. Measuring rhythmic spatial contraction of the myocardium is crucial for assessing mechanical durability and the ability to mount coordinated responses to pressure, electrical, and hemodynamic signals. However, current cardiomyocyte measurement platforms typically focus on action potentials and XY-plane contractions. Therefore, effective evaluation methods for studying the influence of mechanical cues on the spatial dynamic contraction of cardiomyocytes are still lacking. In this study, we developed a topographic guiding combined with an optical spatial motion tracking method to provide controllable mechanical stimulation for inducing directed contraction of cardiomyocytes and obtaining spatial motion information in vitro. We first performed a detailed investigation of cell connections and cytoskeleton orientations by combining the proposed method with immunofluorescence. Next, spatial constrictive modes, features, and key parameters of microgroove-guided cardiomyocytes were studied. Finally, the three-dimensional (3D) motions of the cardiomyocytes at different positions on the structure were compared. We found that the XY-plane contraction of cardiomyocytes typically has only one direction and shows a significant phase delay compared to the axial motion. In addition, cardiomyocytes located near the edges of the microgrooves were restricted by stronger mechanical forces, resulting in a significant height change reduction. These results provide new perspectives for structural and functional research on cardiomyocytes under long-term mechanical regulation. Overall, this study provides a highly precise and convenient method for evaluating the 3D cardiomyocyte motion under mechanical induction. This method is expected to enhance understanding of cardiomyocyte development and be useful for research on cardiac mechanics and functions.

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测量受机械线索调控的心肌细胞空间运动行为的新型非侵入式方法
完整的心脏会经历复杂的多尺度机械重塑过程。测量心肌的节律性空间收缩对于评估机械耐久性以及对压力、电和血流动力学信号做出协调反应的能力至关重要。然而,目前的心肌细胞测量平台通常侧重于动作电位和 XY 平面收缩。因此,目前仍缺乏有效的评估方法来研究机械线索对心肌细胞空间动态收缩的影响。在这项研究中,我们开发了一种结合光学空间运动跟踪方法的地形引导,提供可控的机械刺激,用于诱导心肌细胞定向收缩并获取体外空间运动信息。我们首先结合免疫荧光法对细胞连接和细胞骨架方向进行了详细研究。接着,研究了微槽引导心肌细胞的空间收缩模式、特征和关键参数。最后,比较了结构上不同位置的心肌细胞的三维(3D)运动。我们发现,心肌细胞的 XY 平面收缩通常只有一个方向,而且与轴向运动相比有明显的相位延迟。此外,位于微槽边缘附近的心肌细胞受到较强机械力的限制,导致高度变化明显减小。这些结果为长期机械调控下的心肌细胞结构和功能研究提供了新的视角。总之,本研究为评估机械诱导下的三维心肌细胞运动提供了一种高度精确和便捷的方法。该方法有望加深对心肌细胞发育的理解,并有助于心脏力学和功能的研究。
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来源期刊
Materials Today Physics
Materials Today Physics Materials Science-General Materials Science
CiteScore
14.00
自引率
7.80%
发文量
284
审稿时长
15 days
期刊介绍: Materials Today Physics is a multi-disciplinary journal focused on the physics of materials, encompassing both the physical properties and materials synthesis. Operating at the interface of physics and materials science, this journal covers one of the largest and most dynamic fields within physical science. The forefront research in materials physics is driving advancements in new materials, uncovering new physics, and fostering novel applications at an unprecedented pace.
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