OsSPL11 positively regulates grain size by activating the expression of GW5L in rice.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES Plant Cell Reports Pub Date : 2024-09-05 DOI:10.1007/s00299-024-03315-7
Xinhai Zeng, Kai Fan, Yu Shi, Rui Chen, Wanyu Liu, Xin Wang, Guixiang Ye, Wenxiong Lin, Zhaowei Li
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Abstract

Key message: Rice OsSPL11 activates the expression of GW5L through binding to its promoter and positively regulates grain size. Grain size (GS) is an important determinant of grain weight and yield potential in cereal. Here, we report the functional analysis of OsSPL11 in grain length (GL), grain width (GW), and 1000-grain weight (TGW). OsSPL11 mutant plants, osspl11 lines, exhibited a decrease in GL, GW, and TGW, and OsSPL11-OE lines showed an increase in GL and TGW. Expression analysis revealed that OsSPL11 was located in the nucleus and highly expressed in spikelet hull and young development grains, consistent with its function in determining GS. Further analysis confirmed that OsSPL11 directly activates the expression of GW5L to regulate GS, meanwhile OsSPL11 expression is negatively regulated by OsGBP3. Taken together, our findings demonstrate that OsSPL11 could be a key regulator of affecting GS during the spikelet hull development and facilitate the process of improving grain yield by GS modification in rice.

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OsSPL11 通过激活水稻中 GW5L 的表达来正向调节粒度。
关键信息水稻 OsSPL11 通过与 GW5L 启动子结合激活 GW5L 的表达,并正向调节谷粒大小。谷粒大小(GS)是谷物粒重和产量潜力的重要决定因素。在此,我们报告了 OsSPL11 在谷粒长度(GL)、谷粒宽度(GW)和千粒重(TGW)中的功能分析。OsSPL11 突变体植株(osspl11 株系)的谷粒长度(GL)、谷粒宽度(GW)和千粒重(TGW)均有所下降,而 OsSPL11-OE 株系的谷粒长度(GL)和千粒重(TGW)则有所上升。表达分析表明,OsSPL11 位于细胞核中,在小穗外壳和幼小发育谷粒中高表达,这与其决定 GS 的功能一致。进一步的分析证实,OsSPL11直接激活GW5L的表达以调控GS,同时OsSPL11的表达受OsGBP3的负调控。综上所述,我们的研究结果表明,OsSPL11可能是影响穗壳发育过程中GS的关键调控因子,有助于通过改变GS来提高水稻的籽粒产量。
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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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