Activation of an atypical plant NLR with an N-terminal deletion initiates cell death at the vacuole.

IF 6.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY EMBO Reports Pub Date : 2024-10-01 Epub Date: 2024-09-06 DOI:10.1038/s44319-024-00240-4
Sruthi Sunil, Simon Beeh, Eva Stöbbe, Kathrin Fischer, Franziska Wilhelm, Aron Meral, Celia Paris, Luisa Teasdale, Zhihao Jiang, Lisha Zhang, Moritz Urban, Emmanuel Aguilar Parras, Thorsten Nürnberger, Detlef Weigel, Rosa Lozano-Duran, Farid El Kasmi
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Abstract

Plants evolve nucleotide-binding leucine-rich repeat receptors (NLRs) to induce immunity. Activated coiled-coil (CC) domain containing NLRs (CNLs) oligomerize and form apparent cation channels promoting calcium influx and cell death, with the alpha-1 helix of the individual CC domains penetrating the plasma membranes. Some CNLs are characterized by putative N-myristoylation and S-acylation sites in their CC domain, potentially mediating permanent membrane association. Whether activated Potentially Membrane Localized NLRs (PMLs) mediate cell death and calcium influx in a similar way is unknown. We uncovered the cell-death function at the vacuole of an atypical but conserved Arabidopsis PML, PML5, which has a significant deletion in its CCG10/GA domain. Active PML5 oligomers localize in Golgi membranes and the tonoplast, alter vacuolar morphology, and induce cell death, with the short N-terminus being sufficient. Mutant analysis supports a potential role of PMLs in plant immunity. PML5-like deletions are found in several Brassicales paralogs, pointing to the evolutionary importance of this innovation. PML5, with its minimal CC domain, represents the first identified CNL utilizing vacuolar-stored calcium for cell death induction.

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激活一种 N 端缺失的非典型植物 NLR 会导致细胞在液泡中死亡。
植物进化出核苷酸结合富亮氨酸重复受体(NLRs)来诱导免疫。活化的含盘绕线圈(CC)结构域的 NLRs(CNLs)会寡聚并形成明显的阳离子通道,促进钙离子流入和细胞死亡,单个 CC 结构域的 alpha-1 螺旋可穿透质膜。一些 CNL 的特征是其 CC 结构域中存在假定的 N-肉豆蔻酰化和 S-酰化位点,从而有可能介导永久性膜关联。活化的潜在膜定位 NLRs(PMLs)是否以类似方式介导细胞死亡和钙离子流入尚不清楚。我们发现了拟南芥一种非典型但保守的 PML(PML5)在液泡中的细胞死亡功能,该 PML5 的 CCG10/GA 结构域有显著缺失。活性 PML5 寡聚体定位在高尔基体膜和调质体中,改变液泡形态并诱导细胞死亡,短 N 端足以诱导细胞死亡。突变体分析支持 PML 在植物免疫中的潜在作用。在几种十字花科植物的旁系亲缘植物中发现了类似于 PML5 的缺失,这表明了这一创新在进化过程中的重要性。PML5 具有最小的 CC 结构域,是第一个利用液泡储存的钙诱导细胞死亡的 CNL。
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来源期刊
EMBO Reports
EMBO Reports 生物-生化与分子生物学
CiteScore
11.20
自引率
1.30%
发文量
267
审稿时长
1 months
期刊介绍: EMBO Reports is a scientific journal that specializes in publishing research articles in the fields of molecular biology, cell biology, and developmental biology. The journal is known for its commitment to publishing high-quality, impactful research that provides novel physiological and functional insights. These insights are expected to be supported by robust evidence, with independent lines of inquiry validating the findings. The journal's scope includes both long and short-format papers, catering to different types of research contributions. It values studies that: Communicate major findings: Articles that report significant discoveries or advancements in the understanding of biological processes at the molecular, cellular, and developmental levels. Confirm important findings: Research that validates or supports existing knowledge in the field, reinforcing the reliability of previous studies. Refute prominent claims: Studies that challenge or disprove widely accepted ideas or hypotheses in the biosciences, contributing to the correction and evolution of scientific understanding. Present null data: Papers that report negative results or findings that do not support a particular hypothesis, which are crucial for the scientific process as they help to refine or redirect research efforts. EMBO Reports is dedicated to maintaining high standards of scientific rigor and integrity, ensuring that the research it publishes contributes meaningfully to the advancement of knowledge in the life sciences. By covering a broad spectrum of topics and encouraging the publication of both positive and negative results, the journal plays a vital role in promoting a comprehensive and balanced view of scientific inquiry. 
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