Does NexoBrid® have antimicrobial properties?

IF 3.2 3区医学 Q2 CRITICAL CARE MEDICINE Burns Pub Date : 2024-06-22 DOI:10.1016/j.burns.2024.06.005

Amal Sharaf , Philip Turpin, Neil Ullyott, Preetha Muthayya

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引用次数: 0

Abstract

We previously published the microbial profile of burn wounds managed with NexoBrid® in Pinderfields Regional Burns Centre, Wakefield, UK. Our results showed no significant changes in bacterial colonisation in burn wounds debrided with NexoBrid®. Previous studies described the antimicrobial properties of bromelain enzyme. To date, the effects of NexoBrid® on microorganisms have not been reported. In this study, a series of lab experiments were conducted to investigate the antimicrobial properties of NexoBrid®. Mueller-Hinton agar plates were pre-treated with NexoBrid® and inoculated with common pathogens after serial dilution. Our results revealed that the pre-treated plates showed reduction in the growth of E. faecalis and S. aureus. No zones of inhibition were observed around NexoBrid® after 18 h of incubation. Where a combination of controls and test solutions were added, no zones of inhibition were seen around the NexoBrid® wells in any of fifty tested organisms. The slightly lower infection rates observed in patients treated with NexoBrid® are more likely to be due to efficient selective debridement of necrotic skin rather than direct antimicrobial action.

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NexoBrid® 是否具有抗菌特性？

我们曾在英国韦克菲尔德的 Pinderfields 区域烧伤中心发表过使用 NexoBrid® 处理烧伤创面的微生物概况。我们的研究结果表明，使用 NexoBrid® 清创的烧伤创面的细菌定植情况没有明显变化。以前的研究描述了菠萝蛋白酶的抗菌特性。迄今为止，NexoBrid® 对微生物的影响尚未见报道。在本研究中，我们进行了一系列实验室实验来研究 NexoBrid® 的抗菌特性。用 NexoBrid® 对穆勒-欣顿琼脂平板进行预处理，并在连续稀释后接种常见病原体。结果表明，预处理过的平板上粪肠球菌和金黄色葡萄球菌的生长量有所减少。培养 18 小时后，NexoBrid® 周围未发现抑制区。在加入对照组和测试溶液的情况下，NexoBrid® 孔周围对 50 种受测生物均未出现抑制区。在使用 NexoBrid® 治疗的患者中观察到的感染率略低，这更可能是由于对坏死皮肤进行了有效的选择性清创，而不是直接的抗菌作用。

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来源期刊

Burns 医学-皮肤病学

CiteScore

4.50

自引率

18.50%

发文量

304

审稿时长

72 days

期刊介绍： Burns aims to foster the exchange of information among all engaged in preventing and treating the effects of burns. The journal focuses on clinical, scientific and social aspects of these injuries and covers the prevention of the injury, the epidemiology of such injuries and all aspects of treatment including development of new techniques and technologies and verification of existing ones. Regular features include clinical and scientific papers, state of the art reviews and descriptions of burn-care in practice. Topics covered by Burns include: the effects of smoke on man and animals, their tissues and cells; the responses to and treatment of patients and animals with chemical injuries to the skin; the biological and clinical effects of cold injuries; surgical techniques which are, or may be relevant to the treatment of burned patients during the acute or reconstructive phase following injury; well controlled laboratory studies of the effectiveness of anti-microbial agents on infection and new materials on scarring and healing; inflammatory responses to injury, effectiveness of related agents and other compounds used to modify the physiological and cellular responses to the injury; experimental studies of burns and the outcome of burn wound healing; regenerative medicine concerning the skin.