The development of silk glands and transcriptome aberration induced by cyantraniliprole in Bombyx mori

IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pesticide Biochemistry and Physiology Pub Date : 2024-09-01 DOI:10.1016/j.pestbp.2024.106111
Xiaohan Liu , Ruinan Qi , Fanchi Li , Minjin Han , Bing Li , Haina Sun
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Abstract

Bombyx mori is an insect species of great economic importance, and its silk gland is a vital organ for the synthesis and secretion of silk protein. However, long-term artificial domestication of B. mori has resulted in high sensitivity to chemical toxins, especially insecticides. Cyantraniliprole (Cya), a second-generation ryanodine receptor modulator insecticide, is widely utilized in agriculture for pest control. In this study, the impact of Cya toxicity on the development of silk glands in the 5th instar larvae of B. mori was assessed using Cya LC5, LC10 and LC20, as well as a starvation treatment group for comparison. Short-term exposure (24 h) to different concentrations of Cya resulted in delayed development of silk glands in B. mori. Meanwhile, the body weight, silk gland weight, silk gland index and cocoon quality were significantly reduced in a concentration-dependent manner, except for the Cya LC5 treatment. Histopathological and ultrastructural analysis revealed that Cya LC10 induced disruption of the nuclear membrane and endoplasmic reticulum in the posterior silk gland (PSG) cells, leading to the formation of intracellular vacuoles. Transcriptome sequencing of PSGs identified 2152 genes that were differentially expressed after exposure to Cya LC10, with 1153 down-regulated genes and 999 up-regulated genes. All differentially expressed genes were subjected to functional annotation using gene ontology and Kyoto encyclopedia of genes and genomes database, and it was found that protein synthesis-related pathways were significantly enriched, with the majority of genes being down-regulated. Furthermore, the transcription levels of genes involved in “protein processing in endoplasmic reticulum”, “protein export”, “proteasome” and “DNA replication” were quantified using qRT-PCR. Our findings suggested that short-term exposure to Cya LC10 resulted in disruption of DNA replication, as well as protein transport, processing and hydrolysis in the PSG cells of B. mori. The results of this study provide a theoretical foundation for the safe utilization of Cya in sericulture production.

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氰戊菊酯诱导的丝腺发育和转录组畸变
桑蚕是一种具有重要经济价值的昆虫,其丝腺是合成和分泌丝蛋白的重要器官。然而,长期的人工驯化导致桑蚕对化学毒素(尤其是杀虫剂)高度敏感。氰虫酰胺(Cya)是第二代雷诺丁受体调节剂杀虫剂,被广泛用于农业害虫防治。在本研究中,使用 Cya LC5、LC10 和 LC20 评估了 Cya 毒性对 B. mori 5龄幼虫丝腺发育的影响,并使用饥饿处理组进行比较。短期接触(24 小时)不同浓度的 Cya 会导致桑蚕丝腺发育延迟。同时,除Cya LC5处理外,体重、丝腺重量、丝腺指数和蚕茧质量均显著降低,且降低程度与浓度有关。组织病理学和超微结构分析表明,Cya LC10可诱导后丝腺细胞核膜和内质网的破坏,导致细胞内空泡的形成。PSG的转录组测序确定了暴露于Cya LC10后有差异表达的2152个基因,其中1153个基因下调,999个基因上调。利用基因本体论和京都基因和基因组百科全书数据库对所有差异表达基因进行了功能注释,发现蛋白质合成相关通路显著富集,大部分基因下调。此外,还利用 qRT-PCR 对涉及 "内质网蛋白质加工"、"蛋白质输出"、"蛋白酶体 "和 "DNA 复制 "的基因的转录水平进行了定量分析。我们的研究结果表明,短期暴露于 Cya LC10 会导致 B. mori PSG 细胞中 DNA 复制以及蛋白质运输、加工和水解的中断。这项研究结果为在养蚕生产中安全使用 Cya 提供了理论基础。
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来源期刊
CiteScore
7.00
自引率
8.50%
发文量
238
审稿时长
4.2 months
期刊介绍: Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.
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