Identification and characterization of a novel Wx-B1 allele in a waxy wheat (Triticum aestivum L.)

IF 1.7 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Plant Biotechnology Reports Pub Date : 2024-09-11 DOI:10.1007/s11816-024-00925-1
Yeonjun Sung, Keonghoon Kim, Jinhee Park, Seongwook Kang, Chulsoo Park, Seongwoo Cho, Changsoo Kim
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Abstract

The composition of starch, which includes amylose and amylopectin, greatly affects the quality and characteristics of flour. The balance between these components is crucial in determining the properties and structure of starch. The waxy (Wx) gene encodes granule-bound starch synthase I (GBSSI), which is responsible for amylose biosynthesis in the endosperm. Gunji-3 was created through a cross between the waxy-type wheat cultivar Shinmichal 1 and the bread wheat cultivar Keumkang. Upon comparing the Wx-B1 allele encoding GBSSI in Gunji-3 with the Wx-B1 allele in Shinmichal 1 and Wx-B1b, a total of four SNPs and one deletion were identified in Gunji-3. Additionally, when comparing amino acid sequences with Wx-B1 alleles, differences at three positions were found, indicating that the mutant carried a new Wx-B1 allele named Wx-B1o. The physicochemical properties of Gunji-3 starch were characterized by a lower amylose content of 2.30% compared to 7.45% in Shinmichal 1 and 27.67% in Keumkang. Additionally, it exhibited a higher water retention capacity of 84.66% compared to 75.91% in Shinmichal 1 and 66.07% in Keumkang. The newly introduced waxy-type wheat could provide an essential basis for understanding wheat's starch characteristics and various breeding programs.

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蜡质小麦(Triticum aestivum L.)中一种新型 Wx-B1 等位基因的鉴定和特征描述
淀粉(包括直链淀粉和支链淀粉)的成分在很大程度上影响着面粉的质量和特性。这些成分之间的平衡对决定淀粉的性质和结构至关重要。蜡质(Wx)基因编码颗粒结合淀粉合成酶 I(GBSSI),它负责胚乳中淀粉的生物合成。Gunji-3 是蜡质型小麦栽培品种 Shinmichal 1 与面包型小麦栽培品种 Keumkang 杂交育成的。将贡二3号中编码GBSSI的Wx-B1等位基因与神麦1号和Wx-B1b中的Wx-B1等位基因进行比较后,在贡二3号中总共发现了四个SNP和一个缺失。此外,在将氨基酸序列与 Wx-B1 等位基因进行比较时,发现在三个位置上存在差异,这表明该突变体带有一个新的 Wx-B1 等位基因,名为 Wx-B1o。贡次3号淀粉的理化性质特点是直链淀粉含量较低,仅为2.30%,而新米查1号和金康淀粉的直链淀粉含量分别为7.45%和27.67%。此外,它的保水率为 84.66%,高于神麦 1 号的 75.91%和金康的 66.07%。新引入的蜡质型小麦可为了解小麦的淀粉特性和各种育种计划提供重要依据。
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来源期刊
Plant Biotechnology Reports
Plant Biotechnology Reports 生物-生物工程与应用微生物
CiteScore
4.10
自引率
4.20%
发文量
72
审稿时长
>12 weeks
期刊介绍: Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.
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