Overexpression of CRK4, the cysteine-rich receptor-like protein kinase of Arabidopsis, regulates the resistance to abiotic stress and abscisic acid responses

IF 1.7 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Plant Biotechnology Reports Pub Date : 2024-09-19 DOI:10.1007/s11816-024-00926-0
Ye-Jin Jang, Sung-Dug Oh, Kangmin Kim, Seong-Kon Lee, Ancheol Chang, Doh-Won Yun, Chul Min Kim, Bumkyu Lee
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Abstract

Receptor-like kinases (RLKs) are known to regulate plant growth, cell differentiation, and defense mechanisms. Cysteine-rich receptor-like protein kinases (CRKs) are a subfamily of RLKs with more than 40 members. In this study, we found that overexpressing CRK4 in Arabidopsis enhances abiotic tolerance to salinity and drought stress. We constructed a CRK4-overexpressed pCB302ES plasmid, transformed it into Arabidopsis and evaluated the insertion site of the transgene. The CRK4-overexpressed line was selected for molecular analyses, including western blotting and flanking T-DNA sequencing. In pots treated with either 200 mM NaCl or dryness, the CRK4-overexpressed line showed higher resistance than the wild-type Columbia-0 (Col-0). The expression levels of DREB2B, RAB18, and RD29B genes associated with plant stress, were lower than those of wild-type Col-0. In addition, ABF1 and abscisic-acid-responsive element-binding factor 1 expression levels increased in CRK4-overexpressed transgenic Arabidopsis. Furthermore, in an abscisic acid (ABA)-containing medium, CRK4-overexpressed transgenic Arabidopsis and wild-type Col-0 showed different root development patterns. Our results indicate that CRK4 modulates the ABA-signaling pathway and responds to salt and drought stress in Arabidopsis. This study helps elucidate the correlation between the ABA and CRK4 genes.

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拟南芥富半胱氨酸受体样蛋白激酶 CRK4 的过表达调控对非生物胁迫的抗性和脱落酸反应
众所周知,类受体激酶(RLKs)可调节植物生长、细胞分化和防御机制。富半胱氨酸受体样蛋白激酶(CRKs)是 RLKs 的一个亚家族,有 40 多个成员。本研究发现,在拟南芥中过表达 CRK4 可增强其对盐度和干旱胁迫的非生物耐受性。我们构建了过表达 CRK4 的 pCB302ES 质粒,将其转化到拟南芥中,并评估了转基因的插入位点。选择CRK4高表达株系进行分子分析,包括西部印迹和侧翼T-DNA测序。在用 200 mM NaCl 或干燥处理的花盆中,CRK4 高表达品系比野生型哥伦比亚-0(Col-0)表现出更高的抗性。与植物胁迫相关的 DREB2B、RAB18 和 RD29B 基因的表达水平低于野生型 Col-0。此外,在 CRK4 表达的转基因拟南芥中,ABF1 和赤霉酸反应元件结合因子 1 的表达水平有所提高。此外,在含脱落酸(ABA)的培养基中,CRK4过表达的转基因拟南芥和野生型Col-0表现出不同的根系发育模式。我们的研究结果表明,CRK4能调节拟南芥的ABA信号通路,并对盐胁迫和干旱胁迫做出响应。这项研究有助于阐明 ABA 和 CRK4 基因之间的相关性。
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来源期刊
Plant Biotechnology Reports
Plant Biotechnology Reports 生物-生物工程与应用微生物
CiteScore
4.10
自引率
4.20%
发文量
72
审稿时长
>12 weeks
期刊介绍: Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.
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