Biochemical Composition and Ecophysiological Characteristics of the Diatom Nitzschia sp. at Different Stages of the Life Cycle

IF 1.1 4区 生物学 Q3 PLANT SCIENCES Russian Journal of Plant Physiology Pub Date : 2024-09-01 DOI:10.1134/s1021443724604762
S. L. Polyakova, S. N. Zheleznova, N. A. Davidovich
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Abstract

The work analyzes the biochemical composition and the influence of some abiotic factors on cells of Nitzschia sp. strains at different stages of the life cycle. The results of the experiment showed the ability of cells to grow in the salinity range of 10–60‰ at the pre-reproductive stage and in the range of 4–60‰ at the reproductive stage which indicates a relatively wide salinity tolerance of the studied species. The optimal salinity (25‰) and illumination (2.95 klx) for a 12-h photoperiod were found to be the same for both parent and daughter cells. The light saturation coefficient for them was 1.4 klx, indicating high energy efficiency of photosynthesis. Analysis of the biochemical composition of two Nitzschia sp. strains in the reproductive and pre-reproductive stages of the life cycle was carried out. During the accumulative cultivation a high content of monoenoic and polyenoic acids was observed. In general, the amount of saturated fatty acids (UFAs) was higher in parental cells, and the amount of monounsaturated fatty acids (MUFAs) was higher in post-initial cells. The quantity of polyunsaturated fatty acids (PUFAs) was almost the same in both cultures. The ω-6/ω-3 ratio showed a predominance of ω-3 fatty acids in both parental and daughter strains. Nitzschia sp. represents an interest for biotechnology given its biochemical composition and fairly high energy efficiency when grown in culture. However, it is extremely important to take into account the phase of the life cycle of the strains in order to obtain individual valuable products.

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硅藻 Nitzschia sp.在生命周期不同阶段的生化组成和生态生理特征
摘要 该研究分析了尼茨藻菌株在生命周期不同阶段的生化组成及一些非生物因素对其细胞的影响。实验结果表明,在生殖前期,细胞能在 10-60‰ 的盐度范围内生长,在生殖期,细胞能在 4-60‰ 的盐度范围内生长,这表明所研究的物种具有相对较宽的耐盐性。研究发现,母细胞和子细胞在 12 小时光周期内的最佳盐度(25‰)和光照度(2.95 klx)是相同的。它们的光饱和系数为 1.4 klx,表明光合作用的能量效率很高。对生命周期生殖阶段和生殖前期的两株尼茨藻的生化成分进行了分析。在累积培养过程中,观察到单烯酸和多烯酸含量较高。一般来说,亲代细胞中饱和脂肪酸(UFAs)的含量较高,而后代细胞中单不饱和脂肪酸(MUFAs)的含量较高。两种培养物中多不饱和脂肪酸(PUFAs)的含量几乎相同。ω-6/ω-3比率显示,亲本和子代菌株中的ω-3脂肪酸占优势。鉴于尼茨藻的生化成分以及在培养过程中相当高的能量效率,尼茨藻对生物技术很有兴趣。然而,为了获得有价值的产品,考虑菌株生命周期的阶段是极其重要的。
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来源期刊
CiteScore
4.00
自引率
14.30%
发文量
107
审稿时长
6 months
期刊介绍: Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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