3-Нydroxypyridine Derivatives Reduce Mitochondrial Dysfunction in Pea Seedlings under Conditions of Water Deficiency

IF 1.1 4区生物学 Q3 PLANT SCIENCES Russian Journal of Plant Physiology Pub Date : 2024-09-01 DOI:10.1134/s1021443724605500

I. V. Zhigacheva, N. I. Krikunova, E. M. Mil, Yu. V. Kuznetsov, I. P. Generozova, P. A. Butsanets, A. N. Goloshchapov

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Abstract

The possibility of using antioxidants-derivatives of 3-hydroxypyridine: N-acetylcysteinate 2-ethyl-6-methyl-3-hydroxypyridine and carnitinate 2-ethyl-6-methyl-3-hydroxypyridine as plant growth regulators investigated on the pea seedlings mitochondria (Pisum sativum L.) Flora 2 cultivar. The influence of water deficiency (WD) and 3-HP on the functional state of mitochondria was studied. Water deficiency activated lipid peroxidation and led to mitochondrial swelling. An increase in LPO intensity was associated with significant changes in the content of C₁₈ fatty acids The content of linoleic and linolenic acids in the mitochondrial membranes of seedlings decreased by 12.5 and 22.3%, respectively. There were also changes in the content of C₂₀ FA: the content of 20:2 ω6 and 20:1 ω9 decreased by almost 1.65 times and 1.5 times, respectively. The swelling of mitochondria, probably, resulted in a partial loss of cytochrome C from the intermembrane space of mitochondria into the cytosol, which led to a decrease in the electron transport rates at the end section of the respiratory chain. The treatment of pea seeds with antioxidants led to the prevention of lipid peroxidation, the prevention changes in the composition of FAs membranes and mitochondrial morphology and also restored the rate of electron transport at the end section of the respiratory chain of these organelles. By preventing lipid peroxidation, the drugs helped maintain the functional state of mitochondria under WD conditions, which probably increased the resistance of pea seedlings to stress.

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3-Нydroxypyridine Derivatives Reduce Mitoondrial Dysfunction in Pea Seedling under Conditions of Water Deficiency豌豆苗在缺水条件下的线粒体功能障碍

AbstractThe possibility of using antioxidants-derivatives of 3-hydroxypyridine：N-acetylcysteinate 2-乙基-6-甲基-3-羟基吡啶和 carnitinate 2-乙基-6-甲基-3-羟基吡啶作为植物生长调节剂对豌豆苗线粒体（Pisum sativum L.) Flora 2 栽培品种进行了研究。研究了缺水（WD）和 3-HP 对线粒体功能状态的影响。缺水激活了脂质过氧化反应，导致线粒体肿胀。幼苗线粒体膜中亚油酸和亚麻酸的含量分别减少了 12.5% 和 22.3%。C20 脂肪酸的含量也发生了变化：20:2 ω6 和 20:1 ω9 的含量分别减少了近 1.65 倍和 1.5 倍。线粒体的膨胀可能导致部分细胞色素 C 从线粒体的膜间隙流失到细胞质中，从而导致呼吸链末端的电子传递速率下降。用抗氧化剂处理豌豆种子可防止脂质过氧化，防止 FAs 膜成分和线粒体形态发生变化，还能恢复这些细胞器呼吸链末端的电子传输速率。通过防止脂质过氧化，这些药物有助于维持线粒体在 WD 条件下的功能状态，这可能增强了豌豆幼苗的抗逆性。

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来源期刊

Russian Journal of Plant Physiology 生物-植物科学

CiteScore

4.00

自引率

14.30%

发文量

107

审稿时长

6 months

期刊介绍： Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.