DEVELOPMENT OF AN IDENTIFICATION METHOD FOR Leishmania spp BASED ON REAL-TIME PCR WITH HIGH-RESOLUTION MELTING

Abstract

Introduction Leishmaniasis is a disease caused by parasites of the genus Leishmania. In Colombia, 6 pathogenic species have been reported. Traditional parasitological diagnosis based on the observation of parasites does not allow the species to be identified, which is why biochemical or molecular methods must be used, including conventional PCR, but this has some limitations and requires long periods of time to obtain results., which are sometimes inconclusive.