Performance Comparison of Four Hepatitis E Antibodies Detection Methods

IF 4.1 2区生物学 Q2 MICROBIOLOGY Microorganisms Pub Date : 2024-09-11 DOI:10.3390/microorganisms12091875

Milagros Muñoz-Chimeno, Nazaret Díaz-Sánchez, Lucía Morago, Vanessa Rodríguez-Paredes, Silvia Barturen, Álvaro Rodríguez-Recio, Maira Alejandra García-Lugo, Maria Isabel Zamora, María Mateo, Mónica Sánchez-Martínez, Ana Avellón

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Abstract

HEV antibody detection constitutes the main screening test for HEV infection. The aim of this study is to compare the sensitivity and specificity of four techniques: LIAISON® MUREX DiaSorin anti-HEV IgG and anti-HEV IgM assays, Hepatitis E VIRCLIA® IgM and IgG monotests, WANTAI HEV-IgM and IgG ELISA and VIDAS® anti-HEV IgM and IgG tests in five panels of samples configurated according to the immunoblot (RecomLine, Mikrogen, Neuss, Germany). Anti-HEV IgM sensitivity in the acute phase was 100% in all techniques, while sensitivity, including the immediate convalescence phase, was 96.74% for LIAISON®, 83.14% for VIRCLIA®, 84.78% for WANTAI and 88.04% for VIDAS®. Anti-HEV IgM specificity was 100% for both LIAISON® and VIRCLIA®. Anti-HEV IgM WANTAI agreed with VIRCLIA® with a good Kappa coefficient (κ = 0.71). Anti-HEV IgG post-infection sensitivity was 100% for LIAISON®, VIDAS® and VIRCLIA® and 99% for WANTAI. Anti-HEV IgG specificity reached 97.17% for LIAISON and 88.68% for VIRCLIA®. Our results demonstrated a better capacity of LIAISON® MUREX anti-HEV IgM than that of competitors for detecting acute infections as well as accurate anti-HEV IgG results and in how to resolve them.

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四种戊型肝炎抗体检测方法的性能比较

HEV 抗体检测是 HEV 感染的主要筛查试验。本研究旨在比较四种技术的灵敏度和特异性：LIAISON® MUREX DiaSorin 抗 HEV IgG 和抗 HEV IgM 检测、戊型肝炎 VIRCLIA® IgM 和 IgG 单项检测、WANTAI HEV-IgM 和 IgG ELISA 以及 VIDAS® 抗 HEV IgM 和 IgG 检测。所有技术在急性期的抗-HEV IgM灵敏度均为100%，而LIAISON®的灵敏度（包括恢复期）为96.74%，VIRCLIA®为83.14%，WANTAI为84.78%，VIDAS®为88.04%。LIAISON® 和 VIRCLIA® 的抗-HEV IgM 特异性均为 100%。抗艾滋病毒 IgM WANTAI 与 VIRCLIA® 的卡帕系数（κ = 0.71）吻合。LIAISON® 、VIDAS® 和 VIRCLIA® 的感染后抗 HEV IgG 敏感性为 100%，WANTAI 为 99%。LIAISON® 和 VIRCLIA® 的抗 HEV IgG 特异性分别为 97.17% 和 88.68%。我们的结果表明，LIAISON® MUREX 抗 HEV IgM 检测急性感染的能力优于同类产品，抗 HEV IgG 检测结果也很准确，并能解决这些问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.