Anti-inflammatory Effect of Symplocos prunifolia Extract in an In Vitro Model of Acute Pneumonia

IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Plant Foods for Human Nutrition Pub Date : 2024-09-10 DOI:10.1007/s11130-024-01231-5
Seok Woo Kim, Wona Jee, So-Mi Park, Ye-Rin Park, Hanbit Bae, Yun-Cheol Na, Han-Gyul Lee, Seungwon Kwon, Hyeung-Jin Jang
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Abstract

Acute pneumonia is a respiratory disease characterized by inflammation within the lung tissue, exhibiting higher morbidity rates and mortality rates among immunocompromised children and older adults. Symplocos species have been traditionally used as herbal remedies for conditions like dysentery, skin ulcers, diarrhea, and dyspepsia. Contemporary research has employed various Symplocos species in the study of diverse diseases. However, the exact efficacy and mechanisms of action of Symplocos Prunifolia remain unknown. Therefore, this study investigated the anti-inflammatory mechanism of S. prunifolia extract (SPE) in A549 and RAW264.7 cells stimulated by lipopolysaccharide (LPS). SPE significantly reduced nitric oxide (NO) production and the protein expression levels of like inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 cells. Furthermore, it reduced the protein expression levels of iNOS, COX-2 and the levels of pro-inflammatory cytokines in LPS-stimulated A549 cells. The mechanism underlying the anti-inflammatory effect of SPE was associated with the inhibition of LPS stimulated the phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) and Mitogen-activated protein kinase (MAPK) phosphorylation. Moreover, we confirmed that SPE decreased the nuclear translocation of nuclear factor-κB (NF-κB)/p65 stimulated by LPS. In conclusion, these results demonstrate that SPE alleviates inflammatory responses by deactivating the PI3K/Akt, MAPK, and NF-κB signaling pathways. Our findings suggest that SPE is a potential candidate for acute pneumonia prevention.

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梅花提取物在急性肺炎体外模型中的抗炎作用
急性肺炎是一种以肺部组织炎症为特征的呼吸道疾病,免疫力低下的儿童和老年人发病率和死亡率较高。传统上,Symplocos 树种被用作治疗痢疾、皮肤溃疡、腹泻和消化不良等疾病的草药。当代的研究已将各种Symplocos物种用于各种疾病的研究。然而,Symplocos Prunifolia 的确切功效和作用机制仍然未知。因此,本研究调查了梅花苷提取物(SPE)在脂多糖(LPS)刺激下对 A549 和 RAW264.7 细胞的抗炎机制。在 LPS 刺激的 RAW 264.7 细胞中,SPE 能明显减少一氧化氮(NO)的产生,并降低诱导型一氧化氮合酶(iNOS)和环氧化酶-2(COX-2)的蛋白表达水平。此外,它还能降低 LPS 刺激的 A549 细胞中 iNOS、COX-2 蛋白表达水平和促炎细胞因子水平。SPE 抗炎作用的机制与抑制 LPS 刺激的磷酸肌醇-3-激酶/蛋白激酶 B(PI3K/Akt)和丝裂原活化蛋白激酶(MAPK)磷酸化有关。此外,我们还证实 SPE 可减少 LPS 刺激的核因子-κB(NF-κB)/p65 的核转位。总之,这些结果表明,SPE 可通过使 PI3K/Akt、MAPK 和 NF-κB 信号通路失活来缓解炎症反应。我们的研究结果表明,SPE 是预防急性肺炎的潜在候选药物。
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来源期刊
Plant Foods for Human Nutrition
Plant Foods for Human Nutrition 工程技术-食品科技
CiteScore
6.80
自引率
7.50%
发文量
89
审稿时长
12-24 weeks
期刊介绍: Plant Foods for Human Nutrition (previously Qualitas Plantarum) is an international journal that publishes reports of original research and critical reviews concerned with the improvement and evaluation of the nutritional quality of plant foods for humans, as they are influenced by: - Biotechnology (all fields, including molecular biology and genetic engineering) - Food science and technology - Functional, nutraceutical or pharma foods - Other nutrients and non-nutrients inherent in plant foods
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