Simultaneous Measurement of Uric Acid, Guanine, and Adenine Using AgNP@Mn-MOFs Composite-Based Electrochemical Sensor at Trace Level: Application to Blood, Urine, and DNA Sample Matrices
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Abstract
A composite containing silver nanoparticles embedded Mn-MOFs has been synthesized using a simple solvothermal route. The composite-modified electrode has been utilized in the simultaneous measurement of purine base pairs of DNA [guanine (GU), adenine (AD)] and uric acid (UA). The morphology of the composite has been studied by scanning electron microscopy which revealed that the Ag nanoparticles homogeneously get distributed over the layers of Mn-MOFs. The thermal stability of the composite has been studied by thermogravimetric analysis. BET adsorption–desorption isotherm study revealed the large surface area and mesoporous nature of the composite. The electrochemical behavior of the composite material has been studied through impedance spectroscopy, cyclic voltammetry (CV), and square wave voltammetry (SWV) techniques to decipher the redox nature of it towards the target analytes like GU, AD, and UA. Each of these analytes has displayed a distinct catalytic oxidative signal with well-resolved peaks during their simultaneous measurement. The linearity obtained for UA, GU, and AD by square wave voltammetry is in the concentration range of 0.5–280 µM with a limit of detection of 64.49, 78.84, and 125.33 nM, respectively. The composite-modified electrode has been successfully applied to real sample matrices like human serum, urine, and commercially available fish sperm DNA samples. The fabricated sensor showed very good responses to these analytes from real sample matrices with prolonged stability and reproducibility.
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