Glabrin from Pongamia pinnata: Structural Insights and Antibacterial Potential

Abstract

Glabrin, a bioactive compound first discovered in Pongamia pinnata seeds has sparked interest for its putative antibacterial potential. In this study, we present a simple and rapid method for the isolation of Glabrin followed by its structural characterization using Density Functional Theory (DFT) and single X-ray crystallography (XRD). Furthermore, in silico and in vitro assessments were conducted to evaluate the antimicrobial nature of Glabrin. Additionally, the mode of antibacterial action was elucidated using Field Emission Scanning Electron Microscopy (FESEM), cell leakage assays, and flow cytometry. Our findings provide insights into the antimicrobial potential of Glabrin and its mechanism of action against bacterial pathogens.