Lignin synthesis pathway in response to Rhizoctonia solani Kühn infection in potato (Solanum tuberosum L.)

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Chemical and Biological Technologies in Agriculture Pub Date : 2024-09-14 DOI:10.1186/s40538-024-00663-0

XinYu Yang, Wangjun Zhang, HePing Lv, YanPing Gao, YiChen Kang, YanBin Wu, FangFang Wang, Wu Zhang, HongJie Liang

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Abstract

Potato black scurf caused by Rhizoctonia solani Kühn is widespread worldwide. The exploration and analysis of the infection mechanism of Rhizoctonia solani Kühn has important scientific significance to enhance the disease resistance of potato and other horticultural crops, and then break the restriction of fungal harm to agricultural production. The physiological and biochemical indexes and the expression levels of related genes were measured at 0, 1, 4, 8 and 16 days (T0, T1, T2, T3, T4) after inoculation with pathogenic bacteria. The results showed that the contents of L-phenylalanine ammonia-lyase (PAL), peroxidase (POD), lignin, total phenols (TP), and flavonoids increased significantly in potato after infection by Rhizoctonia solani Kühn, with the contents of PAL and POD reaching a peak at 8 d and then decreasing, and the contents of lignin and total phenols changing most significantly, reaching the highest levels at day 8 (T3) and day 16 (T4), respectively. During the infestation, the content of eight phenolic compounds increased, and the genes responsible for the lignin synthesis pathway were upregulated. However, in the later stage of infestation, the expression of two genes (PAL PG0031457 and PG2021549, HCT PG0014959, and COMT PG0011266) was down-regulated. In the correlation analysis, gene expression levels of all the genes, except POD (PG0005062), CCoAOMT (PG0018688), and COMT (PG0011266), were found to be positively correlated with the contents of lignin, total phenols, flavonoids, PAL, POD, and eight phenolic substances. Therefore, based on a sound understanding of the occurrence mechanism of Potato black scurf, this experiment analyzed the effect of Rhizoctonia solani Kühn infestation on the content of relevant metabolites in the lignin synthesis pathway as well as gene expression in potatoes, which provides a scientific basis for the prevention and control management of potato black scurf.

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马铃薯（Solanum tuberosum L.）木质素合成途径对根瘤菌（Rhizoctonia solani Kühn ）感染的反应

根瘤菌（Rhizoctonia solani Kühn）引起的马铃薯黑孢病在世界范围内普遍存在。探索和分析Rhizoctonia solani Kühn的侵染机理，对提高马铃薯等园艺作物的抗病性，进而打破真菌对农业生产危害的限制具有重要的科学意义。在病原菌接种后的 0、1、4、8 和 16 天（T0、T1、T2、T3、T4）测定了马铃薯的生理生化指标和相关基因的表达水平。结果表明，马铃薯在受到 Rhizoctonia solani Kühn 感染后，L-苯丙氨酸氨解酶（PAL）、过氧化物酶（POD）、木质素、总酚（TP）和类黄酮的含量显著增加，其中 PAL 和 POD 的含量在 8 d 达到峰值后下降，木质素和总酚的含量变化最大，分别在第 8 天（T3）和第 16 天（T4）达到最高水平。在侵染期间，八种酚类化合物的含量增加，木质素合成途径的基因上调。但在侵染后期，两个基因（PAL PG0031457 和 PG2021549、HCT PG0014959 和 COMT PG0011266）的表达下调。在相关性分析中发现，除 POD（PG0005062）、CCoAOMT（PG0018688）和 COMT（PG0011266）外，其他基因的表达水平均与木质素、总酚、黄酮、PAL、POD 和八种酚类物质的含量呈正相关。因此，本实验在充分认识马铃薯黑皮病发生机理的基础上，分析了Rhizoctonia solani Kühn侵染对马铃薯木质素合成途径中相关代谢产物含量及基因表达的影响，为马铃薯黑皮病的防控管理提供了科学依据。

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.