3D printed gelatin methacryloyl hydrogels for perfusion culture of human trabecular meshwork cells and glaucoma studies

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioengineering Pub Date : 2024-09-18 DOI:10.1002/bit.28849

Bikram Adhikari, Prasanga Barakoti, Mina B. Pantcheva, Melissa D. Krebs

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Abstract

Glaucoma, a progressive eye disease leading to irreversible blindness, currently affects over 70 million people globally. Elevated intraocular pressure (IOP) is implicated in its development. IOP is carefully regulated by the trabecular meshwork (TM). However, studying TM behavior has been limited to traditional tissue culture studies or costly ex vivo cultures of animal and donor eyes. Developing novel functional TM models could enhance cell/tissue behavior understanding and aid therapeutic development for glaucoma. In this study, we 3D printed a simplified and reproducible model of the human TM (hTM) and studied hTM cell behavior under static and dynamic cultures. Gelatin Methacryloyl bioinks proved suitable for printing with viable and proliferative hTM cells expressing crucial marker genes in response to glucocorticoid induction. This, to our knowledge, is the first functional 3D printed hTM model and aims to facilitate TM research. Moreover, this easily reproducible model could also be applicable in the study of numerous other cell types throughout the body.

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用于人类小梁网细胞灌注培养和青光眼研究的三维打印明胶甲基丙烯酰水凝胶

青光眼是一种渐进性眼病，会导致不可逆转的失明，目前全球有 7000 多万人患有青光眼。眼压升高与青光眼的发病有关。眼压受小梁网（TM）的严格调节。然而，对小梁啮合行为的研究一直局限于传统的组织培养研究或昂贵的动物和供体眼球体外培养。开发新型功能性小梁网模型可以加深对细胞/组织行为的理解，有助于青光眼的治疗开发。在这项研究中，我们用三维打印技术打印了一个简化的、可重复的人类 TM（hTM）模型，并研究了 hTM 细胞在静态和动态培养下的行为。事实证明，明胶甲基丙烯酰生物链接适合打印有活力和增殖的 hTM 细胞，这些细胞在糖皮质激素诱导下表达重要的标记基因。据我们所知，这是首个功能性三维打印 hTM 模型，旨在促进 TM 研究。此外，这种易于复制的模型还可用于研究全身其他多种细胞类型。

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

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