Urea-Loaded PLGA Microspheres as Chemotaxis Stimulants for Helicobacter pylori.

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioengineering Pub Date : 2024-11-03 DOI:10.1002/bit.28870
Prasanth Shanmughan, Pravin Subrahmaniyan, Dhruv Bhatnagar, Srinithi Ranganathan, Pushkar P Lele
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Abstract

Helicobacter pylori cells undergo chemotaxis toward several small molecules, called chemo-attractants, including urea produced by the epithelial cells of the stomach. The biophysical mechanisms of chemotaxis are not well understood in H. pylori. Here, we developed point sources of urea by encapsulating it in Poly(lactic-co-glycolic acid) or PLGA microbeads for H. pylori chemotaxis studies. Microscopy and Dynamic Light Scattering characterization indicated that the PLGA particles had an average diameter of < 0.8 μm. The particles were relatively stable and had a net negative surface charge. Absorbance measurements indicated that the beads released ~70% of the urea over a 2-week period, with most of the release occurring within the first 24-h period. Varying pH (2.0-7.0) had little effect on the rate of urea release. A diffusion model predicted that such beads could generate sufficient urea gradients to chemotactically attract H. pylori cells. Single-bead single-cell chemotaxis assays confirmed the predictions, revealing that H. pylori continued to be attracted to beads even after most of the urea had been released in the first 24 h. Our work highlights a novel use of PLGA microbeads as delivery vehicles for stimulating a chemotaxis response in H. pylori, with potential applications in bacterial eradication strategies.

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作为幽门螺旋杆菌趋化刺激剂的尿素负载聚乳酸聚乳酸(PLGA)微球
幽门螺杆菌细胞对几种小分子(称为趋化吸引剂)具有趋化作用,其中包括胃上皮细胞产生的尿素。幽门螺杆菌趋化的生物物理机制尚不十分清楚。在此,我们通过将尿素封装在聚乳酸-共-乙醇酸或聚乳酸-丙烯酸微珠中,开发了尿素点源,用于幽门螺杆菌趋化研究。显微镜和动态光散射表征表明,PLGA 颗粒的平均直径为
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来源期刊
Biotechnology and Bioengineering
Biotechnology and Bioengineering 工程技术-生物工程与应用微生物
CiteScore
7.90
自引率
5.30%
发文量
280
审稿时长
2.1 months
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