{"title":"Field-portable digital holographic quantitative phase imaging with a compact microscope's add-on module","authors":"Samira Ebrahimi , Masoomeh Dashtdar , Carlo Fonda , Humberto Cabrera","doi":"10.1016/j.optlaseng.2024.108580","DOIUrl":null,"url":null,"abstract":"<div><p>Digital holographic microscopy (DHM) is emerged as a promising quantitative phase-contrast imaging tool for full complex wavefront reconstruction of micron-sized bio-samples. The technique covers the dynamics investigation ranging in scales from sub-cellular to tissue and from milliseconds to hours. Recent advances of DHM lie in the configuration and numerical development of the method and making it more feasible for the users without optical expertise. In this paper, we aim to propose a low-cost and portable add-on module for DHM, which can be mounted on either the ocular or camera port of a conventional microscope and easily turn it to a multi-modal bright-field and DHM imaging tool. The module works based on the off-axis, common-path geometry using a single Fresnel biprism in the detection path of the microscope. This configuration enables a compact and cost-effective solution for point of care applications and in field measurements. The feasibility and efficiency of the device have been confirmed through several morphological investigations on biological specimens and the sub-nanometer phase stability enables the measurement of cell dynamics and phenotypic changes such as motility, growth, differentiation and membrane oscillations.</p></div>","PeriodicalId":49719,"journal":{"name":"Optics and Lasers in Engineering","volume":"184 ","pages":"Article 108580"},"PeriodicalIF":3.5000,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optics and Lasers in Engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S014381662400558X","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 0
Abstract
Digital holographic microscopy (DHM) is emerged as a promising quantitative phase-contrast imaging tool for full complex wavefront reconstruction of micron-sized bio-samples. The technique covers the dynamics investigation ranging in scales from sub-cellular to tissue and from milliseconds to hours. Recent advances of DHM lie in the configuration and numerical development of the method and making it more feasible for the users without optical expertise. In this paper, we aim to propose a low-cost and portable add-on module for DHM, which can be mounted on either the ocular or camera port of a conventional microscope and easily turn it to a multi-modal bright-field and DHM imaging tool. The module works based on the off-axis, common-path geometry using a single Fresnel biprism in the detection path of the microscope. This configuration enables a compact and cost-effective solution for point of care applications and in field measurements. The feasibility and efficiency of the device have been confirmed through several morphological investigations on biological specimens and the sub-nanometer phase stability enables the measurement of cell dynamics and phenotypic changes such as motility, growth, differentiation and membrane oscillations.
期刊介绍:
Optics and Lasers in Engineering aims at providing an international forum for the interchange of information on the development of optical techniques and laser technology in engineering. Emphasis is placed on contributions targeted at the practical use of methods and devices, the development and enhancement of solutions and new theoretical concepts for experimental methods.
Optics and Lasers in Engineering reflects the main areas in which optical methods are being used and developed for an engineering environment. Manuscripts should offer clear evidence of novelty and significance. Papers focusing on parameter optimization or computational issues are not suitable. Similarly, papers focussed on an application rather than the optical method fall outside the journal''s scope. The scope of the journal is defined to include the following:
-Optical Metrology-
Optical Methods for 3D visualization and virtual engineering-
Optical Techniques for Microsystems-
Imaging, Microscopy and Adaptive Optics-
Computational Imaging-
Laser methods in manufacturing-
Integrated optical and photonic sensors-
Optics and Photonics in Life Science-
Hyperspectral and spectroscopic methods-
Infrared and Terahertz techniques