Central cholinergic transmission modulates endocannabinoid-induced marble-burying behavior in mice

IF 2.6 3区 心理学 Q2 BEHAVIORAL SCIENCES Behavioural Brain Research Pub Date : 2024-09-13 DOI:10.1016/j.bbr.2024.115252
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Abstract

Central cholinergic system and endocannabinoid, anandamide exhibits anti-compulsive-like behavior in mice. However, the role of the central cholinergic system in the anandamide-induced anti-compulsive-like behavior is still unexplored. Therefore, the present study assessed the role of central cholinergic transmission in the anandamide-induced anti-compulsive activity using a marble-burying behavior (MBB) model in mice. The modulation in the anandamide-induced effect on MBB was evaluated using mice with altered central cholinergic transmission achieved by pretreatment (i.c.v.) with various cholinergic agents like acetylcholine (ACh), acetylcholinesterase inhibitor (AChEI), neostigmine, nicotine, mAChR antagonist, atropine, and nAChR antagonist, mecamylamine. The influence of anandamide treatment on the brain AChE activity was also evaluated. The results revealed that i.c.v. injection of anandamide (10, 20 µg/mouse, i.c.v.) dose-dependently reduced MBB in mice. Moreover, anandamide in all the tested doses inhibited the brain AChE activity indicating the role of an enhanced central cholinergic transmission in its anti-compulsive-like effect . Furthermore, the anti-compulsive-like effect of anandamide (20 µg/mouse, i.c.v.) was found to be enhanced in mice centrally pre-treated with, ACh (0.1 µg/mouse, i.c.v.) or AChEI, neostigmine (0.3 µg/mouse, i.c.v.). In addition, the anandamide-induced anti-compulsive-like effect was significantly increased in mice pre-treated with a low dose of nicotine (0.1 µg/mouse, i.c.v.) while, it was attenuated by the higher dose of nicotine (2 µg/mouse, i.c.v.). On the other hand, the anandamide (20 µg/mouse, i.c.v.) induced anti-compulsive-like effect was found to be diminished in mice pre-treated with mAChR antagonist, atropine (0.1, 0.5 µg/mouse, i.c.v.) and pre-injection of nAChR antagonist, mecamylamine (0.1, 0.5 µg/mouse, i.c.v.) potentiated the anandamide induced anti-compulsive-like response in mice. Thus, the present investigation delineates the modulatory role of an enhanced central cholinergic transmission in the anandamide-induced anti-compulsive-like behavior in mice by inhibition of brain AChE or via muscarinic and nicotinic receptors mediated mechanism.

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中枢胆碱能传导调节内源性大麻素诱导的小鼠埋大理石行为
中枢胆碱能系统和内源性大麻素--安乃近在小鼠身上表现出反强迫行为。然而,中枢胆碱能系统在安乃近诱导的类强迫行为中的作用仍未得到探索。因此,本研究利用小鼠埋大理石行为(MBB)模型评估了中枢胆碱能传导在安乃近诱导的反强迫活动中的作用。通过对小鼠进行各种胆碱能药物如乙酰胆碱(ACh)、乙酰胆碱酯酶抑制剂(AChEI)、新斯的明、尼古丁、mAChR拮抗剂阿托品和nAChR拮抗剂甲氰咪胍的预处理(静脉注射),评估了安乃近诱导的对大理石掩埋行为的影响。此外,还评估了安乃近处理对脑 AChE 活性的影响。结果显示,静脉注射安乃近(10、20 微克/只小鼠,静脉注射)可剂量依赖性地降低小鼠的 MBB。此外,所有测试剂量的安乃近都能抑制大脑 AChE 的活性,这表明安乃近的抗强迫样作用增强了中枢胆碱能传导。此外,安乃近(20 微克/只小鼠,静注)的抗强迫样作用在使用 ACh(0.1 微克/只小鼠,静注)或 AChEI、新斯的明(0.3 微克/只小鼠,静注)进行中枢预处理的小鼠中得到增强。此外,用低剂量尼古丁(0.1微克/只小鼠,静注)预处理的小鼠,其安乃近诱导的抗强迫样作用明显增强,而用高剂量尼古丁(2微克/只小鼠,静注)预处理的小鼠,其作用则减弱。另一方面,用 mAChR 拮抗剂阿托品(0.1、0.5微克/只小鼠,静注)和预先注射nAChR拮抗剂麦卡米拉明(0.1,0.5微克/只小鼠,静注)可增强小鼠的安乃近诱导的抗强迫样反应。因此,本研究通过抑制脑 AChE 或通过毒蕈碱受体和烟碱受体介导的机制,阐明了增强的中枢胆碱能传导在安乃近诱导的小鼠反强迫样行为中的调节作用。
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来源期刊
Behavioural Brain Research
Behavioural Brain Research 医学-行为科学
CiteScore
5.60
自引率
0.00%
发文量
383
审稿时长
61 days
期刊介绍: Behavioural Brain Research is an international, interdisciplinary journal dedicated to the publication of articles in the field of behavioural neuroscience, broadly defined. Contributions from the entire range of disciplines that comprise the neurosciences, behavioural sciences or cognitive sciences are appropriate, as long as the goal is to delineate the neural mechanisms underlying behaviour. Thus, studies may range from neurophysiological, neuroanatomical, neurochemical or neuropharmacological analysis of brain-behaviour relations, including the use of molecular genetic or behavioural genetic approaches, to studies that involve the use of brain imaging techniques, to neuroethological studies. Reports of original research, of major methodological advances, or of novel conceptual approaches are all encouraged. The journal will also consider critical reviews on selected topics.
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