Enhanced Therapeutic Potential of Liposome-Coated Bushen Jianpi Recipe for Hepatocellular Carcinoma

IF 2.8 4区医学 Q2 PHARMACOLOGY & PHARMACY Current drug delivery Pub Date : 2024-09-18 DOI:10.2174/0115672018318595240902095514

Siqi Feng, Yi Zhong, Yabin Li, Shiying Li, Yun Li, Zhangjie Zhou, Zhonghua Wu, Tingting Wu

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Abstract

Objective: Hepatocellular carcinoma (HCC) poses a major healthcare burden globally. Traditional Chinese medicine formula Bushen Jianpi (BSJP) recipe shows inhibitory effects on HCC but suffers from low bioavailability. This study aims to develop a BSJP-loaded liposome (BSJP@Lip) for targeted HCC treatment. Methods: BSJP@Lip was prepared using a microfluidic device. Particle characterization included size, morphology, drug loading, encapsulation efficiency, and release kinetics analysis. In vitro cytotoxicity, cellular uptake, apoptosis, and protein expression were evaluated in hepG2, Smmc-7721, and hepa 1-6 hepatic cancer cell lines treated with BSJP@Lip. Results: BSJP@Lip nanoparticles showed a uniform spherical shape with an average size of 50 nm and zeta potential at around -2.24 mV. They significantly inhibited cell viability and induced apoptosis in a dose-dependent manner compared with traditional decoction formulations. Enhanced cellular uptake of BSJP@Lip increased the expression of proinflammatory factors IL-18 and NLRP3. Conclusion: BSJP@Lip nanoparticles were found to be efficiently internalized by hepatic cancer cell lines, resulting in a dose-dependent inhibition of cell viability and induction of apoptosis. This effect was accompanied by the upregulation of IL-18 and NLRP3.

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增强脂质体包裹的釜陈陈皮配方对肝细胞癌的治疗潜力

目的：肝细胞癌（HCC）是全球主要的医疗负担。传统中药配方布参建皮（BSJP）对 HCC 有抑制作用，但生物利用度较低。本研究旨在开发一种用于靶向治疗 HCC 的 BSJP 脂质体（BSJP@Lip）。研究方法使用微流控装置制备 BSJP@Lip。颗粒表征包括尺寸、形态、载药量、包封效率和释放动力学分析。在用 BSJP@Lip 处理的 hepG2、Smmc-7721 和 hepa 1-6 肝癌细胞系中，对体外细胞毒性、细胞摄取、细胞凋亡和蛋白表达进行了评估。结果显示BSJP@Lip 纳米粒子呈均匀球形，平均粒径为 50 nm，zeta 电位约为 -2.24 mV。与传统的煎煮制剂相比，BSJP@Lip 纳米颗粒能以剂量依赖性的方式明显抑制细胞活力并诱导细胞凋亡。增强细胞对 BSJP@Lip 的吸收会增加促炎因子 IL-18 和 NLRP3 的表达。结论研究发现，BSJP@Lip 纳米颗粒可被肝癌细胞株有效内化，从而导致剂量依赖性的细胞活力抑制和细胞凋亡诱导。这种效应伴随着 IL-18 和 NLRP3 的上调。

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来源期刊

Current drug delivery PHARMACOLOGY & PHARMACY-

CiteScore

5.10

自引率

4.20%

发文量

170

期刊介绍： Current Drug Delivery aims to publish peer-reviewed articles, research articles, short and in-depth reviews, and drug clinical trials studies in the rapidly developing field of drug delivery. Modern drug research aims to build delivery properties of a drug at the design phase, however in many cases this idea cannot be met and the development of delivery systems becomes as important as the development of the drugs themselves. The journal aims to cover the latest outstanding developments in drug and vaccine delivery employing physical, physico-chemical and chemical methods. The drugs include a wide range of bioactive compounds from simple pharmaceuticals to peptides, proteins, nucleotides, nucleosides and sugars. The journal will also report progress in the fields of transport routes and mechanisms including efflux proteins and multi-drug resistance. The journal is essential for all pharmaceutical scientists involved in drug design, development and delivery.