A biocompatible nanoformulation of curcumin analogue and curd exosomes targeting EphA2 signalling cascade in head and neck cancer

IF 4.5 2区 工程技术 Q2 NANOSCIENCE & NANOTECHNOLOGY Cancer Nanotechnology Pub Date : 2024-09-18 DOI:10.1186/s12645-024-00286-y
Kaumudi Pande, B. K. Bettadaiah, Anbarasu Kannan
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Abstract

Major therapeutic developments have been made in the prevention of head and neck cancer (HNC), and crucial measures have been implemented for the survival of patients. The advent of cancer nano-theranostic as an effective approach targets cancer by allowing drug aggregation at the tumour site, its proper bioaccessibility, and tumour cell death. Curd exosomes are the cellular interactive nanovesicles, considered a convenient conveyance medium for cargoes still unexplored. Curcumin analogue alanine is primarily recognised for its superior radical scavenging activity and anti-mutagen properties compared with curcumin. The current study focussed on the isolation and characterisation of curd exosomes, followed by their interaction with cancer cells to deliver their content conveniently. Herein, we developed a nanoformulation of curd exosomes loaded with curcumin alanine to determine its bioaccessibility and anti-proliferative effect compared with curcumin alanine free drug. In addition, the influence of curcumin alanine and its nanoformulation on cell morphology, nucleus structures, colony formation potential, and tumour cell death was observed. The expression of EphA2 and its associated molecules was determined using western blot and PCR to explore the mechanism at the cellular level. The recent investigation revealed the encapsulation of curcumin analogue alanine in curd exosomes enhanced the bioaccessibility in contrast with curcumin alanine. Then, we focussed on the curcumin alanine effect on HNC cells to monitor morphological alterations, a reduction in cell multiplication, and triggering apoptosis. Particularly, we found considerable suppression of EphA2 influencing mitochondrial dynamics with the strengthening of mitochondrial fusion MFN1 and MFN2, whereas fission-associated protein DRP1 was down-regulated by the treatment of curcumin alanine nanoformulation. Furthermore, curcumin alanine nanoformulation activates the apoptotic marker caspase-7 and suppresses the anti-apoptotic marker Bcl-xL. Hence, these findings have drawn attention to curd exosomes loaded curcumin alanine nanoformulation impairing cell multiplication and mitochondrial fission, leading to apoptotic cell death, as one of the effective approaches for the treatment of HNC.
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针对头颈癌 EphA2 信号级联的姜黄素类似物和凝乳外泌体的生物相容性纳米制剂
在预防头颈癌(HNC)的治疗方面取得了重大进展,并为患者的生存采取了重要措施。癌症纳米疗法的出现是一种有效的方法,它通过使药物在肿瘤部位聚集、适当的生物可及性和肿瘤细胞死亡来治疗癌症。凝乳外泌体是一种与细胞相互作用的纳米微粒,被认为是一种方便的货物运输介质,但目前仍未被开发。与姜黄素相比,姜黄素类似物丙氨酸具有更强的自由基清除活性和抗突变特性。目前的研究重点是分离和鉴定凝乳外泌体,然后将其与癌细胞相互作用,以方便地传递其中的成分。在此,我们开发了一种负载姜黄素丙氨酸的凝乳外泌体纳米制剂,与不含姜黄素丙氨酸的药物相比,确定其生物可及性和抗增殖效果。此外,还观察了姜黄素丙氨酸及其纳米制剂对细胞形态、细胞核结构、集落形成潜能和肿瘤细胞死亡的影响。研究人员还利用 Western 印迹和 PCR 技术测定了 EphA2 及其相关分子的表达,以探索其在细胞水平的作用机制。最近的研究发现,与姜黄素丙氨酸相比,将姜黄素类似物丙氨酸包裹在凝乳外泌体中可提高生物可及性。随后,我们重点研究了姜黄素丙氨酸对 HNC 细胞的影响,以监测细胞形态的改变、细胞繁殖的减少以及细胞凋亡的触发。特别是,我们发现姜黄素丙氨酸纳米制剂可显著抑制影响线粒体动力学的EphA2,增强线粒体融合蛋白MFN1和MFN2,而裂变相关蛋白DRP1则在姜黄素丙氨酸纳米制剂的处理下被下调。此外,姜黄素丙氨酸纳米制剂还能激活凋亡标志物 Caspase-7 并抑制抗凋亡标志物 Bcl-xL。因此,这些发现引起了人们对姜黄素丙氨酸纳米化的关注,即姜黄素外泌体负载姜黄素丙氨酸纳米化可损害细胞增殖和线粒体分裂,导致细胞凋亡,是治疗 HNC 的有效方法之一。
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来源期刊
Cancer Nanotechnology
Cancer Nanotechnology Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
5.20
自引率
1.80%
发文量
37
审稿时长
15 weeks
期刊介绍: Aim: Recognizing cancer as a group of diseases caused by nanostructural problems (i.e. with DNA) and also that there are unique benefits to approaches inherently involving nanoscale structures and processes to treat the disease, the journal Cancer Nanotechnology aims to disseminate cutting edge research; to promote emerging trends in the use of nanostructures and the induction of nanoscale processes for the prevention, diagnosis, treatment of cancer; and to cover related ancillary areas. Scope: Articles describing original research in the use of nanostructures and the induction of nanoscale processes for the prevention, diagnosis and treatment of cancer (open submission process). Review, editorial and tutorial articles picking up on subthemes of emerging importance where nanostructures and the induction of nanoscale processes are used for the prevention, diagnosis and treatment of cancer.
期刊最新文献
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