James C Pearce, Amy Housden, Nicola J Senior, Olivia L Champion, Joann L Prior, Richard W Titball, James G Wakefield
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Abstract
A limitation to the non-vertebrate 3Rs model Galleria mellonella has been the lack of genetic toolkit. A common requirement for genetic tractability is a method to introduce exogenous material to the unicellular embryo, the most common of which is microinjection. This short article describes a detailed method for rearing Galleria mellonella to collect large amounts of staged embryos and to dechorionate and microinject embryos with limited mortality.
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