Inkjet-printed graphene multielectrode arrays: an accessible platform for in vitro cardiac electrophysiology

Jairo Lumpuy-Castillo, Yujie Fu, Alan Avila, Kateryna Solodka, Jiantong Li, Oscar Lorenzo, Erica Zeglio, Leonardo Daniel Garma
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Abstract

In vitro models have now become a realistic alternative to animal models for cardiotoxicity assessment. However, the cost and expertise required to implement in vitro electrophysiology systems to study cardiac cells poses a strong obstacle to their widespread use. This study presents a novel, cost-effective approach for in vitro cardiac electrophysiology using fully-printed graphene-based microelectrode arrays (pGMEAs) coupled with an open-source signal acquisition system. We characterized the pGMEAs' electrical properties and biocompatibility, observing low impedance values and cell viability. We demonstrated the platform's capability to record spontaneous electrophysiological activity from HL-1 cell cultures, and we monitored and quantified their responses to chemical stimulation with noradrenaline. This study demonstrates the feasibility of producing fully-printed, graphene-based devices for in vitro electrophysiology. The accessible and versatile platform we present here represents a step further in the development of alternative methods for cardiac safety screening.
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喷墨打印石墨烯多电极阵列:体外心脏电生理学的便捷平台
体外模型现已成为心脏毒性评估动物模型的现实替代方案。然而,实施体外电生理学系统研究心脏细胞所需的成本和专业知识对其广泛应用构成了巨大障碍。本研究介绍了一种新颖、经济高效的体外心脏电生理学方法,该方法使用完全印刷的石墨烯基微电极阵列(pGMEAs)和开源信号采集系统。我们对 pGMEAs 的电特性和生物相容性进行了鉴定,观察到了低阻抗值和细胞存活率。我们展示了该平台记录 HL-1 细胞培养物自发电生理活动的能力,并监测和量化了它们对去甲肾上腺素化学刺激的反应。这项研究证明了生产基于石墨烯的全印刷体外电生理学设备的可行性。我们在此介绍的这个易用且多功能的平台代表着我们在开发心脏安全性筛选的替代方法方面又迈进了一步。
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