The MYB transcription factor PpMYB5 regulates Pp4CL1/Pp4CL2 expression to promote lignin biosynthesis of fruit russeting in the flat nectarine

IF 5.3 2区生物学 Q1 PLANT SCIENCES Plant Cell Reports Pub Date : 2024-09-14 DOI:10.1007/s00299-024-03321-9

Yule Miao, Wenyi Duan, Ang Li, Mingzhu Yuan, Junren Meng, Hongmei Wang, Lei Pan, Shihang Sun, Guochao Cui, Caiyun Shi, Liang Niu, Wenfang Zeng

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Key message

Transcription factor PpMYB5 promotes lignin synthesis by directly binding to the Pp4CL1/Pp4CL2 promoter and affecting their expression, which may be related to nectarine russeting formation.

Abstract

Nectarine russeting is usually considered to be a non-invasive physiological disease that usually occurs on late-maturing cultivars and seriously affects their appearance quality and commercial value. The cause of nectarine fruit rust is currently unknown. In this study, we compared two flat nectarine cultivars, ‘zhongyoupanweidi’ (HD; russeting-free cultivar) and ‘zhongyoupanweihou’ (TH; russeting-prone cultivar), with respect to nectarine russeting by means of microscopy, transcriptomics, and hormone analysis. Compared to HD fruits, TH fruits had a broken cuticle, missing wax layer, and heavy lignin deposition. RNA sequencing (RNA-seq) revealed significant alternations in the expression of genes related to lignin synthesis. Moreover, structure genes Pp4CL1 and Pp4CL2, MYB transcription factor (TF) gene PpMYB5 were identified through weighted gene co-expression network analysis (WGCNA). Molecular experiments and transgenic evidence suggested that PpMYB5 regulates Pp4CL1/Pp4CL2 expression to promote lignin synthesis. Overall, in addition to providing new insights into the formation of mechanisms for nectarine russeting, our study also establishes a foundation for nectarine russeting prevention.

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MYB 转录因子 PpMYB5 调控 Pp4CL1/Pp4CL2 的表达，促进扁平油桃果实赤皮的木质素生物合成

关键信息转录因子PpMYB5通过直接与Pp4CL1/Pp4CL2启动子结合并影响其表达来促进木质素的合成，这可能与油桃赤霉病的形成有关。摘要油桃赤霉病通常被认为是一种非侵染性生理性病害，通常发生在晚熟栽培品种上，严重影响其外观品质和商业价值。油桃果锈病的病因目前尚不清楚。在本研究中，我们通过显微镜、转录组学和激素分析，比较了两个扁平油桃栽培品种'zhongyoupanweidi'（HD，无赤霉病栽培品种）和'zhongyoupanweihou'（TH，易赤霉病栽培品种）在油桃赤霉病方面的情况。与 HD 果实相比，TH 果实的角质层破损，蜡层缺失，木质素沉积严重。RNA 测序（RNA-seq）显示，与木质素合成相关的基因表达发生了显著变化。此外，通过加权基因共表达网络分析（WGCNA）发现了结构基因 Pp4CL1 和 Pp4CL2、MYB 转录因子（TF）基因 PpMYB5。分子实验和转基因证据表明，PpMYB5 可调控 Pp4CL1/Pp4CL2 的表达，从而促进木质素的合成。总之，我们的研究除了为油桃赤霉病的形成机制提供了新的见解外，还为预防油桃赤霉病奠定了基础。

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来源期刊

Plant Cell Reports 生物-植物科学

CiteScore

10.80

自引率

1.60%

发文量

135

审稿时长

3.2 months

期刊介绍： Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.