Enhancer of Shoot Regeneration 2 (ESR2) regulates pollen maturation and vitality in watermelon (Citrullus lanatus)

IF 4.6 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of Integrative Agriculture Pub Date : 2024-05-31 DOI:10.1016/j.jia.2024.05.032
Hu Wang, Lihong Cao, Yalu Guo, Zheng Li, Huanhuan Niu
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Abstract

Watermelon () holds global significance as a fruit with high economic and nutritional value. Exploring the regulatory network of watermelon male reproductive development is crucial for developing male sterile materials and facilitating cross-breeding. Despite its importance, there is a lack of research on the regulation mechanism of male reproductive development in watermelon. In this study, we identified that , a VIIIb subclass member in the (/) superfamily, was a key factor in pollen development. RNA hybridization confirmed significant expression in the tapetum and pollen during the later stage of anther development. The pollens of transgenic plants showed major defects in morphology and vitality at the late development stage. The RNA-seq and protein interaction assay confirmed that regulates pollen morphology and fertility by interacting with key genes involved in pollen development at both transcriptional and protein levels. These suggest that Enhancer of Shoot Regeneration 2 (ESR2) plays an important role in pollen maturation and vitality. This study helps understand the male reproductive development of watermelon, providing a theoretical foundation for developing male sterile materials.
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嫩枝再生促进因子 2(ESR2)调控西瓜(Citrullus lanatus)花粉的成熟和活力
西瓜()作为一种具有很高经济和营养价值的水果在全球具有重要意义。探索西瓜雄性生殖发育的调控网络对于开发雄性不育材料和促进杂交育种至关重要。尽管西瓜雄性生殖发育非常重要,但对其调控机制的研究却十分缺乏。在本研究中,我们发现(/)超家族中的 VIIIb 亚类成员是花粉发育的关键因素。RNA 杂交证实,在花药发育的后期阶段,Ⅴ在绦膜和花粉中有显著表达。转基因植株的花粉在发育后期出现了严重的形态和活力缺陷。RNA-seq 和蛋白质相互作用分析证实,转基因植物通过在转录和蛋白质水平上与参与花粉发育的关键基因相互作用,调控花粉形态和繁殖力。这表明,嫩枝再生促进因子 2(ESR2)在花粉成熟和活力方面发挥着重要作用。这项研究有助于了解西瓜雄性生殖发育过程,为开发雄性不育材料提供理论基础。
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来源期刊
Journal of Integrative Agriculture
Journal of Integrative Agriculture AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
7.90
自引率
4.20%
发文量
4817
审稿时长
3-6 weeks
期刊介绍: Journal of Integrative Agriculture publishes manuscripts in the categories of Commentary, Review, Research Article, Letter and Short Communication, focusing on the core subjects: Crop Genetics & Breeding, Germplasm Resources, Physiology, Biochemistry, Cultivation, Tillage, Plant Protection, Animal Science, Veterinary Science, Soil and Fertilization, Irrigation, Plant Nutrition, Agro-Environment & Ecology, Bio-material and Bio-energy, Food Science, Agricultural Economics and Management, Agricultural Information Science.
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