Polymorphism in the Drug Transporter Gene ABCB1 as a Potential Disease Modifier in Cortisol-Producing Adrenal Adenomas

IF 1.6 4区 医学 Q4 ENDOCRINOLOGY & METABOLISM Experimental and Clinical Endocrinology & Diabetes Pub Date : 2024-09-18 DOI:10.1055/a-2408-0718
Frederick Vogel, Leah Braun, Sharmilee Vetrivel, Ru Zhang, Stephanie Zopp, Andrea Oßwald, Elisabeth Nowak, Katharina Schilbach, Martin Bidlingmaier, Petra Zimmermann, Felix Beuschlein, Michaela Hartmann, Stefan Wudy, Anna Riester, Martin Reincke
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Abstract

Introduction Endogenous hypercortisolism presents with variable phenotypes. Etiological factors accounting for the level of hypercortisolism or varying severity of associated comorbidities are lacking. Recently, the adrenal ATP-binding cassette B1 (ABCB1) gene was identified as a modulator of glucocorticoid secretion.

Objective To evaluate the effect of ABCB1 polymorphism rs2032582 on steroid metabolome and clinical phenotypes in patients with endogenous hypercortisolism.

Methods In this cross-sectional cohort study, 137 patients prospectively enrolled in the German Cushing’s registry were included (41 with ACTH-producing pituitary adenoma, 21 with cortisol-producing adrenal adenoma, and 75 with excluded hypercortisolism). In all patients, ABCB1 polymorphism was analyzed using a TaqMan genotyping assay, glucocorticoid metabolite excretion in 24-hour urine samples was analyzed by gas chromatography-mass spectrometry, and the clinical phenotype was assessed systematically.

Results In patients with cortisol-producing adrenal adenomas, but not in patients with ACTH-producing pituitary adenomas, homozygous major allele GG of ABCB1 polymorphism rs2032582 was associated with higher overall cortisol metabolite secretion (median 13515 [IQR 10347; 25669] µg/24h vs. 9645 [6146; 10732] µg/24h in minor homo- and heterozygotes, p=0.036) and elevated major cortisol metabolites αTHF, THF and THE (9339 [6929; 17789] µg/24h vs. 6288 [4184; 7455] µg/24h, p=0.045). Moreover, these patients showed higher mean arterial pressure (116 [111; 131] mmHg in major homozygotes vs. 105 [96; 112] mmHg in minor homo- and heterozygotes, p=0.036).

Conclusion The genotype of drug transporter gene ABCB1 rs2032582 polymorphism is associated with the degree of cortisol metabolite secretion in cortisol-producing adrenal adenomas and could, therefore, represent a modifier of disease severity in this context.

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药物转运基因 ABCB1 的多态性是皮质醇分泌型肾上腺腺瘤的潜在疾病调节因子
引言 内源性皮质醇过多症表现出不同的表型。导致皮质醇分泌过多或相关合并症严重程度不同的病因尚不明确。最近,肾上腺 ATP 结合盒 B1(ABCB1)基因被确认为糖皮质激素分泌的调节因子。目的 评估 ABCB1 多态性 rs2032582 对内源性皮质醇增多症患者类固醇代谢组和临床表型的影响。方法 在这项横断面队列研究中,纳入了德国库欣病登记处前瞻性登记的 137 例患者(41 例为促肾上腺皮质激素分泌垂体腺瘤患者,21 例为皮质醇分泌肾上腺腺瘤患者,75 例为排除性高皮质醇症患者)。采用 TaqMan 基因分型检测法分析了所有患者的 ABCB1 多态性,采用气相色谱-质谱法分析了 24 小时尿样中糖皮质激素代谢物的排泄情况,并对临床表型进行了系统评估。 结果 在皮质醇分泌型肾上腺腺瘤患者中,但在 ACTH 分泌型垂体腺瘤患者中,ABCB1 多态性 rs2032582 的同源主等位基因 GG 与较高的皮质醇代谢物总分泌量相关(中位数 13515 [IQR 10347; 25669] µg/24h vs. 中位数 9645 [6146; 10669] µg/24h vs. 中位数 25669 µg/24h vs. 中位数 25669 µg/24h)。9645[6146;10732]微克/24小时,p=0.036)和主要皮质醇代谢物αTHF、THF和THE的升高(9339[6929;17789]微克/24小时 vs. 6288[4184;7455]微克/24小时,p=0.045)有关。此外,这些患者的平均动脉压也较高(主要同卵双生者为 116 [111; 131] mmHg,次要同卵双生者和杂合子为 105 [96; 112] mmHg,P=0.036)。 结论 药物转运基因 ABCB1 rs2032582 多态性的基因型与产生皮质醇的肾上腺腺瘤分泌皮质醇代谢物的程度有关,因此,在这种情况下,它可能是疾病严重程度的调节因子。
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来源期刊
CiteScore
4.10
自引率
5.60%
发文量
72
审稿时长
3 months
期刊介绍: Publishing outstanding articles from all fields of endocrinology and diabetology, from molecular biology to clinical research, this journal is a brilliant resource. Since being published in English in 1983, the popularity of this journal has grown steadily, reflecting the importance of this publication within its field. Original contributions and short communications appear in each issue along with reviews addressing current topics. In addition, supplementary issues are published each year presenting abstracts or proceedings of national and international scientific meetings. The journal was initially published in German and is still the oldest endocrinological periodical in the German-language market!
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