Efficient conversion of xylan to l-arabinose by multi-enzymatic cascade reaction including d-xylulose 4-epimerase as a new stereoselectivity-exchange enzyme
{"title":"Efficient conversion of xylan to l-arabinose by multi-enzymatic cascade reaction including d-xylulose 4-epimerase as a new stereoselectivity-exchange enzyme","authors":"","doi":"10.1016/j.biortech.2024.131502","DOIUrl":null,"url":null,"abstract":"<div><p><span>l</span>-Arabinose has been produced by hydrolyzing arabinan, a component of hemicellulose. However, <span>l</span>-arabinose has limitations in industrial applications owing to its relatively high cost. Here, <span>d</span>-xylulose 4-epimerase as a new-type enzyme was developed from <span>d</span>-tagaturonate 3-epimerase from <em>Thermotoga petrophila</em> using structure-guided enzyme engineering. <span>d</span>-Xylulose 4-epimerase, which epimerized <span>d</span>-xylulose to <span>l-</span>ribulose, <span>d</span>-xylulokinase and sugar phosphatase, which overcame the equilibrium of <span>d</span>-xylose isomerase, were included to establish a new efficient conversion pathway from <span>d</span>-xylose to <span>l</span>-arabinose. <span>l</span>-Arabinose at 34 g/L was produced from 100 g/L xylan in 45 h by multi-enzymatic cascade reaction using xylanase and enzymes involved in the established conversion pathway. As <span>l</span>-ribulokinase was used instead of <span>d</span>-xylulokinase in the established conversion pathway, an efficient reverse-directed conversion pathway from <span>l</span>-arabinose to <span>d</span>-xylose and the production of <span>d</span>-xylose from arabinan using arabinanase and enzymes involved in the proposed pathway are proposed.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7000,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0960852424012069","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}
引用次数: 0
Abstract
l-Arabinose has been produced by hydrolyzing arabinan, a component of hemicellulose. However, l-arabinose has limitations in industrial applications owing to its relatively high cost. Here, d-xylulose 4-epimerase as a new-type enzyme was developed from d-tagaturonate 3-epimerase from Thermotoga petrophila using structure-guided enzyme engineering. d-Xylulose 4-epimerase, which epimerized d-xylulose to l-ribulose, d-xylulokinase and sugar phosphatase, which overcame the equilibrium of d-xylose isomerase, were included to establish a new efficient conversion pathway from d-xylose to l-arabinose. l-Arabinose at 34 g/L was produced from 100 g/L xylan in 45 h by multi-enzymatic cascade reaction using xylanase and enzymes involved in the established conversion pathway. As l-ribulokinase was used instead of d-xylulokinase in the established conversion pathway, an efficient reverse-directed conversion pathway from l-arabinose to d-xylose and the production of d-xylose from arabinan using arabinanase and enzymes involved in the proposed pathway are proposed.
期刊介绍:
Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies.
Topics include:
• Biofuels: liquid and gaseous biofuels production, modeling and economics
• Bioprocesses and bioproducts: biocatalysis and fermentations
• Biomass and feedstocks utilization: bioconversion of agro-industrial residues
• Environmental protection: biological waste treatment
• Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.