Molecular analysis of canine distemper virus H gene in the golden jackal (Canis aureus) population from Serbia.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES BMC Veterinary Research Pub Date : 2024-09-21 DOI:10.1186/s12917-024-04284-5
Dimitrije Glišić, Milica Kuručki, Duško Ćirović, Sofija Šolaja, Jovan Mirčeta, Vesna Milićević
{"title":"Molecular analysis of canine distemper virus H gene in the golden jackal (Canis aureus) population from Serbia.","authors":"Dimitrije Glišić, Milica Kuručki, Duško Ćirović, Sofija Šolaja, Jovan Mirčeta, Vesna Milićević","doi":"10.1186/s12917-024-04284-5","DOIUrl":null,"url":null,"abstract":"<p><p>Canine distemper virus (CDV) is a highly contagious and often fatal disease affecting wild and domesticated carnivores. The virus is a single-stranded RNA virus from the genus Morbillivirus and the family Paramyxoviridae. While domestic dogs are the most common hosts, the virus poses a significant threat to endangered wildlife due to its broad host range. This study aimed to characterize the CDV Haemagglutinin (H) gene in golden jackals and explore the molecular evolution of the virus in an underrepresented host. A total of 88 brain samples from hunted golden jackals were tested for the presence of CDV viral nucleic acid, and the H gene of positive samples was amplified and sequenced using the Sanger method. Phylogenetic analysis, conducted using maximum likelihood methods, revealed that all Serbian sequences clustered within the Arctic lineage. Notably, the analysis identified a tyrosine (Y) at position 549 of the H protein, a mutation commonly associated with wildlife hosts, instead of the histidine (H) typically found in domestic strains. Additionally, a mutation at position 310 was observed, which could potentially affect the protein's function and virus-host interactions. These findings provide valuable insights into the genetic diversity and evolutionary dynamics of CDV in golden jackals, with broader implications for understanding the virus's adaptability to different hosts. Further research is needed to investigate the functional impact of these mutations, particularly their role in vaccine efficacy and disease transmission across wildlife and domestic species.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"426"},"PeriodicalIF":2.3000,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415988/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Veterinary Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1186/s12917-024-04284-5","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Canine distemper virus (CDV) is a highly contagious and often fatal disease affecting wild and domesticated carnivores. The virus is a single-stranded RNA virus from the genus Morbillivirus and the family Paramyxoviridae. While domestic dogs are the most common hosts, the virus poses a significant threat to endangered wildlife due to its broad host range. This study aimed to characterize the CDV Haemagglutinin (H) gene in golden jackals and explore the molecular evolution of the virus in an underrepresented host. A total of 88 brain samples from hunted golden jackals were tested for the presence of CDV viral nucleic acid, and the H gene of positive samples was amplified and sequenced using the Sanger method. Phylogenetic analysis, conducted using maximum likelihood methods, revealed that all Serbian sequences clustered within the Arctic lineage. Notably, the analysis identified a tyrosine (Y) at position 549 of the H protein, a mutation commonly associated with wildlife hosts, instead of the histidine (H) typically found in domestic strains. Additionally, a mutation at position 310 was observed, which could potentially affect the protein's function and virus-host interactions. These findings provide valuable insights into the genetic diversity and evolutionary dynamics of CDV in golden jackals, with broader implications for understanding the virus's adaptability to different hosts. Further research is needed to investigate the functional impact of these mutations, particularly their role in vaccine efficacy and disease transmission across wildlife and domestic species.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
塞尔维亚金豺种群中犬瘟病毒 H 基因的分子分析。
犬瘟热病毒(CDV)是一种影响野生和驯化食肉动物的高度传染性疾病,通常会导致死亡。该病毒是 Morbillivirus 属 Paramyxoviridae 科的一种单链 RNA 病毒。虽然家犬是最常见的宿主,但由于其宿主范围广泛,该病毒对濒危野生动物构成了重大威胁。本研究旨在确定 CDV 血凝素(H)基因在金毛豺中的特性,并探索该病毒在代表性不足的宿主中的分子进化。本研究共检测了 88 份被猎杀的金毛豺脑样本,以检测是否存在 CDV 病毒核酸,并采用 Sanger 方法对阳性样本的 H 基因进行了扩增和测序。使用最大似然法进行的系统发生分析表明,所有塞尔维亚序列都属于北极系。值得注意的是,分析发现在 H 蛋白的 549 位有一个酪氨酸(Y)突变,而不是家畜菌株中常见的组氨酸(H)突变,这是野生动物宿主常见的突变。此外,在第 310 位还发现了一个突变,这可能会影响蛋白质的功能和病毒与宿主的相互作用。这些发现为了解金毛豺CDV的遗传多样性和进化动态提供了宝贵的见解,对理解病毒对不同宿主的适应性具有更广泛的意义。还需要进一步研究这些突变的功能影响,特别是它们在疫苗效力和疾病在野生动物和家畜物种间传播中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
期刊最新文献
Clinical presentation, investigation findings, and treatment outcomes of intraluminal small intestinal obstruction by bezoars and other materials in adult cows- a retrospective study of 110 cases. Infectious keratoconjunctivitis in European bison (Bison bonasus) in Poland: risk factors, epidemiology and anatomopathological changes with analysis of potential role of Thelazia nematodes in the disease development. Mycoplasma synoviae elongation factor thermo stable is an adhesion-associated protein that enters cells by endocytosis and stimulates DF-1 cell proliferation. Survey in ruminants from Rwanda revealed high diversity and prevalence of extended-spectrum cephalosporin-resistant Enterobacterales. First report of transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) in pigs from Poland.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1