Loss-of-function mutation in anthocyanidin reductase activates the anthocyanin synthesis pathway in strawberry.

IF 10.6 Q1 HORTICULTURE Molecular Horticulture Pub Date : 2024-09-14 DOI:10.1186/s43897-024-00106-2
Pengbo Xu, Maobai Li, Chao Ma, Xinyu Li, Peng Bai, Anqi Lin, Chong Wang, Liqing Zhang, Huiyun Kuang, Hongli Lian
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Abstract

Fruit color substantially affects consumer preferences, with darker red strawberries being economically more valuable due to their higher anthocyanin content. However, the molecular basis for the dark red coloration remains unclear. Through screening of an ethyl methanesulfonate mutant library, we identified a rg418 mutant, that demonstrated anthocyanin accumulation during early fruit development stages. Furthermore, the ripening fruits of this mutant had higher anthocyanin content than wild-type (WT) fruits. An analysis of flavonoid content in WT and rg418 mutant fruits revealed substantial changes in metabolic fluxes, with the mutant exhibiting increased levels of anthocyanins and flavonols and decreased levels of proanthocyanidins. Bulked sergeant analysis sequencing indicated that the mutant gene was anthocyanidin reductase (ANR), a key gene in the proanthocyanidin synthesis pathway. Furthermore, transcriptome sequencing revealed the increased expression of MYB105 during the early development stage of mutant fruits, which promoted the expression of UFGT (UDP-glucose flavonoid 3-O-glucosyltransferase), a key gene involved in anthocyanin synthesis, thus substantially enhancing the anthocyanin content in the mutant fruits. Additionally, mutating ANR in a white-fruited strawberry variant (myb10 mutant) resulted in appealing pink-colored fruits, suggesting the diverse roles of ANR in fruit color regulation. Our study provides valuable theoretical insights for improving strawberry fruit color.

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花青素还原酶功能缺失突变激活草莓花青素合成途径
水果的颜色对消费者的喜好有很大影响,深红色草莓由于花青素含量较高,经济价值更高。然而,深红色的分子基础仍不清楚。通过筛选甲磺酸乙酯突变体文库,我们发现了一个 rg418 突变体,该突变体在果实早期发育阶段表现出花青素积累。此外,该突变体成熟果实的花青素含量高于野生型果实。对WT和rg418突变体果实中类黄酮含量的分析表明,代谢通量发生了很大变化,突变体的花青素和黄酮醇含量增加,原花青素含量减少。大块军士分析测序表明,突变基因是花青素还原酶(ANR),它是原花青素合成途径中的一个关键基因。此外,转录组测序发现,在突变体果实的早期发育阶段,MYB105的表达量增加,促进了参与花青素合成的关键基因UFGT(UDP-葡萄糖类黄酮3-O-葡萄糖基转移酶)的表达,从而大幅提高了突变体果实中的花青素含量。此外,在白果草莓变体(myb10突变体)中突变ANR,可获得诱人的粉红色果实,这表明ANR在果实颜色调节中发挥着多种作用。我们的研究为改善草莓果实颜色提供了宝贵的理论依据。
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来源期刊
Molecular Horticulture
Molecular Horticulture horticultural research-
CiteScore
8.00
自引率
0.00%
发文量
24
审稿时长
12 weeks
期刊介绍: Aims Molecular Horticulture aims to publish research and review articles that significantly advance our knowledge in understanding how the horticultural crops or their parts operate mechanistically. Articles should have profound impacts not only in terms of high citation number or the like, but more importantly on the direction of the horticultural research field. Scope Molecular Horticulture publishes original Research Articles, Letters, and Reviews on novel discoveries on the following, but not limited to, aspects of horticultural plants (including medicinal plants): ▪ Developmental and evolutionary biology ▪ Physiology, biochemistry and cell biology ▪ Plant-microbe and plant-environment interactions ▪ Genetics and epigenetics ▪ Molecular breeding and biotechnology ▪ Secondary metabolism and synthetic biology ▪ Multi-omics dealing with data sets of genome, transcriptome, proteome, metabolome, epigenome and/or microbiome. The journal also welcomes research articles using model plants that reveal mechanisms and/or principles readily applicable to horticultural plants, translational research articles involving application of basic knowledge (including those of model plants) to the horticultural crops, novel Methods and Resources of broad interest. In addition, the journal publishes Editorial, News and View, and Commentary and Perspective on current, significant events and topics in global horticultural fields with international interests.
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