The two-component histidine kinase BdHk1 regulates fungal development, virulence and fungicide sensitivity in Botryosphaeria dothidea

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pesticide Biochemistry and Physiology Pub Date : 2024-09-17 DOI:10.1016/j.pestbp.2024.106134

Meiqi Zhu , Tinghua Huan , Yanru Ma, Yang Han, Na Liu, Sen Lian, Baohua Li, Weichao Ren

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Abstract

Histidine kinases (HKs) allow fungal cells to sense and respond to environmental stimuli. However, the biological role of HKs in Botryosphaeria dothidea, the causal agent of Botryosphaeria canker and apple ring rot, remains unknown. In this study, we identified and characterized the two-component histidine kinase BdHk1 in B. dothidea. Targeted knockout of BdHK1 gene resulted in severe conidiation and pathogenicity defects. In addition, the ΔBdHk1 mutant showed hypersensitivity to osmotic stress, but resistance to phenylpyrrole and dicarboximide fungicides. Moreover, the ΔBdHk1 mutant exhibited significantly increased sensitivity to the cell membrane-damaging agent SDS and high temperature. Comparative transcriptome analysis revealed that inactivation of BdHk1 influenced multiple metabolic pathways in B. dothidea. Taken together, our results suggest that BdHk1 plays an important role in development, virulence and stress tolerance in B. dothidea.

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组氨酸双组分激酶 BdHk1 调节多毛孢霉的真菌发育、毒力和杀菌剂敏感性

组氨酸激酶（HKs）使真菌细胞能够感知环境刺激并做出反应。然而，组氨酸激酶在苹果腐烂病和苹果环腐病的病原菌 Botryosphaeria dothidea 中的生物学作用仍然未知。在这项研究中，我们发现并鉴定了多刺胞螨中的双组分组氨酸激酶 BdHk1。BdHK1 基因的靶向敲除导致了严重的分生和致病性缺陷。此外，ΔBdHk1 突变体对渗透胁迫过敏，但对苯吡咯和二甲酰亚胺类杀菌剂有抗性。此外，ΔBdHk1 突变体对细胞膜破坏剂 SDS 和高温的敏感性显著增加。比较转录组分析表明，BdHk1 的失活影响了 B. dothidea 的多种代谢途径。综上所述，我们的研究结果表明，BdHk1 在 B. dothidea 的发育、毒力和抗逆性中发挥着重要作用。

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.