Delayed jamming-induced oscillatory migration patterns of epithelial collectives under long-range confinement.

IF 1.5 4区 生物学 Q4 CELL BIOLOGY Integrative Biology Pub Date : 2024-01-23 DOI:10.1093/intbio/zyae016
S Lohmann, F M Pramotton, A Taloni, A Ferrari, D Poulikakos, C Giampietro
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Abstract

Collective dynamics of cells in confined geometry regulate several biological processes including cell migration, proliferation, differentiation, and communication. In this work, combining simulation with experimental data, we studied the oscillatory motion of epithelial sheets in smaller areas of confinement, and we linked the monolayer maturation induced-jamming with the wave formation. We showed that epithelial cell populations with delayed jamming properties use the additional time available from this delay to coordinate their movement, generating wave motion in larger areas of confinement compared to control populations. Furthermore, the effects of combining geometric confinement with contact guiding micro-gratings on this wave formation were investigated. We demonstrated that collective migratory oscillations under large geometrical confinement depend on the jamming state of the cell monolayers. The early dynamical state of the experimental results obtained was simulated by self-propelled Voronoi computations, comparing cells with solid-like and fluid-like behavior. Together our model describes the wave formation under confinement and the nodal oscillatory dynamics of the early dynamic stage of the system. Insight Box: Collective behavior of cells in confined spaces impacts biological processes. Through experimental data combined with simulations, the oscillatory motion of epithelial sheets in small areas of confinement was described. A correlation between the level of cell jamming and the formation of waves was detected. Cell populations with delayed jamming presented wave motion in larger confinement areas. The effects of combining geometric confinement with substrate micro-gratings demonstrated that the collective migratory oscillations in large confinement areas rely on the jamming state of cells. The early dynamical state was simulated using self-propelled Voronoi computations that help to understand wave formation under confinement and the nodal oscillatory dynamics of early-stage systems.

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长程限制下上皮细胞集体的延迟干扰诱导振荡迁移模式。
细胞在封闭几何形状中的集体动力学调节着多个生物过程,包括细胞迁移、增殖、分化和交流。在这项工作中,我们结合模拟和实验数据,研究了上皮细胞片在较小的封闭区域内的振荡运动,并将单层成熟诱导的干扰与波的形成联系起来。我们发现,具有延迟干扰特性的上皮细胞群利用这种延迟带来的额外时间来协调它们的运动,与对照群相比,它们在更大的封闭区域内产生了波浪运动。此外,我们还研究了几何限制与接触引导微光栅相结合对这种波形成的影响。我们证明,大几何限制下的集体迁移振荡取决于细胞单层的干扰状态。我们通过自走式 Voronoi 计算模拟了实验结果的早期动力学状态,比较了具有类固体和类流体行为的细胞。我们的模型描述了在封闭条件下的波形成和系统早期动态阶段的节点振荡动力学。洞察框:细胞在密闭空间中的集体行为会影响生物过程。通过实验数据与模拟相结合,描述了上皮细胞片在小范围密闭空间中的振荡运动。研究发现了细胞干扰程度与波的形成之间的相关性。具有延迟干扰的细胞群在较大的封闭区域内呈现波浪运动。几何限制与基底微栅格相结合的效果表明,大限制区域内的集体迁移振荡依赖于细胞的干扰状态。利用自走式 Voronoi 计算模拟了早期动力学状态,这有助于理解禁闭下的波形成和早期系统的节点振荡动力学。
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来源期刊
Integrative Biology
Integrative Biology 生物-细胞生物学
CiteScore
4.90
自引率
0.00%
发文量
15
审稿时长
1 months
期刊介绍: Integrative Biology publishes original biological research based on innovative experimental and theoretical methodologies that answer biological questions. The journal is multi- and inter-disciplinary, calling upon expertise and technologies from the physical sciences, engineering, computation, imaging, and mathematics to address critical questions in biological systems. Research using experimental or computational quantitative technologies to characterise biological systems at the molecular, cellular, tissue and population levels is welcomed. Of particular interest are submissions contributing to quantitative understanding of how component properties at one level in the dimensional scale (nano to micro) determine system behaviour at a higher level of complexity. Studies of synthetic systems, whether used to elucidate fundamental principles of biological function or as the basis for novel applications are also of interest.
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