Effect of bioceramic inclusions on gel-cast aliphatic polymer membranes for bone tissue engineering applications: An in vitro study.

IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Bio-medical materials and engineering Pub Date : 2024-09-26 DOI:10.3233/BME-240079
Vasudev Vivekanand Nayak, Edmara T P Bergamo, Vijayavenkataraman Sanjairaj, Rakesh Kumar Behera, Nikhil Gupta, Paulo G Coelho, Lukasz Witek
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Abstract

Background: Polylactic acid (PLA) has been extensively used in tissue engineering. However, poor mechanical properties and low cell affinity have limited its pertinence in load bearing bone tissue regeneration (BTR) devices.

Objective: Augmenting PLA with β-Tricalcium Phosphate (β-TCP), a calcium phosphate-based ceramic, could potentially improve its mechanical properties and enhance its osteogenic potential.

Methods: Gels of PLA and β-TCP were prepared of different % w/w ratios through polymer dissolution in acetone, after which polymer-ceramic membranes were synthesized using the gel casting workflow and subjected to characterization.

Results: Gel-cast polymer-ceramic constructs were associated with significantly higher osteogenic capacity and calcium deposition in differentiated osteoblasts compared to pure polymer counterparts. Immunocytochemistry revealed cell spreading over the gel-cast membrane surfaces, characterized by trapezoidal morphology, distinct rounded nuclei, and well-aligned actin filaments. However, groups with higher ceramic loading expressed significantly higher levels of osteogenic markers relative to pure PLA membranes. Rule of mixtures and finite element models indicated an increase in theoretical mechanical strength with an increase in β-TCP concentration.

Conclusion: This study potentiates the use of PLA/β-TCP composites in load bearing BTR applications and the ability to be used as customized patient-specific shape memory membranes in guided bone regeneration.

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生物陶瓷夹杂物对用于骨组织工程的凝胶铸造脂肪族聚合物膜的影响:体外研究。
背景:聚乳酸(PLA)已被广泛应用于组织工程。然而,较差的机械性能和较低的细胞亲和性限制了其在承重骨组织再生(BTR)装置中的相关性:目的:用β-磷酸三钙(β-TCP)(一种磷酸钙基陶瓷)增强聚乳酸有可能改善其机械性能并增强其成骨作用:方法:通过丙酮溶解聚合物,制备出不同重量比的聚乳酸和β-TCP凝胶,然后采用凝胶浇注工作流程合成聚合物陶瓷膜并进行表征:结果:与纯聚合物相比,凝胶浇铸聚合物陶瓷构建体的成骨能力和分化成骨细胞中的钙沉积明显更强。免疫细胞化学显示,细胞在凝胶浇铸膜表面铺展,具有梯形形态、明显的圆形细胞核和排列整齐的肌动蛋白丝。然而,与纯聚乳酸膜相比,陶瓷负载较高的组表达的成骨标记物水平明显更高。混合物和有限元模型的规则表明,随着β-TCP浓度的增加,理论机械强度也会增加:这项研究提高了聚乳酸/β-TCP 复合材料在承重 BTR 应用中的使用潜力,以及在引导骨再生中用作定制的患者特异性形状记忆膜的能力。
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来源期刊
Bio-medical materials and engineering
Bio-medical materials and engineering 工程技术-材料科学:生物材料
CiteScore
1.80
自引率
0.00%
发文量
73
审稿时长
6 months
期刊介绍: The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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