Nanozyme linked multi-array gas driven sensor for real-time quantitative detection of Group A streptococcus†

IF 3.6 3区 化学 Q2 CHEMISTRY, ANALYTICAL Analyst Pub Date : 2024-10-08 DOI:10.1039/D4AN00787E
Qi Wang, Pei Liu, Ke Xiao, Wenying Zhou, Jinfeng Li and Yun Xi
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Abstract

Group A streptococcus (GAS) is a pathogen typically transmitted through respiratory droplets and skin contact, causing an estimated 700 million mild non-invasive infections worldwide each year. There are approximately 650 000 infections that progress to severe invasive infections, even resulting in death. Therefore, the ability to detect GAS rapidly, accurately and in real time is important. Herein, we developed a nanozyme linked multi-array gas driven sensor (NLMAGS) to point-of-care testing of GAS within 2 h. The NLMAGS demonstrated excellent performance as it combined the advantages of nanozyme techniques, immunoassay techniques, and 3D printing techniques. Platinum- and palladium-rich nanozyme particles (Au@Pt@PdNPs) were synthesized and used to label monocloning antibodies as detection probes. Magnetic beads were labeled with monocloning antibodies as capture probes to establish a double-antibody sandwich immunoassay for the detection of GAS. The sandwich immune complex can catalyze the H2O2 substrate and produce O2. GAS quantification can be achieved by measuring the distance that the O2 pushes the ink drops forward in the sensor. Under optimized conditions, the NLMAGS quantitatively detected 24 spiked samples with a limit of detection (LOD) of 62 CFU mL−1, which was 5 times lower than that of ELISA (334 CFU mL−1). A strong correlation with the conventional ELISA was found (r = 0.99, P < 0.001). In comparison, the traditional lateral flow immunoassay based on Au@Pt@PdNPs-mAb2 (Au@Pt@PdNPs-LFIA) had a LOD of 104 CFU mL−1, which was significantly higher than that of NLMAGS. The NLMAGS demonstrated excellent sensitivity to GAS. The intra- and inter-assay precisions of the sensor were below 15%. Overall, the established NLMAGS has promising potential as a rapid and quantitative method for detecting GAS and can also be used to detect various pathogens.

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用于实时定量检测 A 群链球菌的纳米酶联接多阵列气体驱动传感器
A 组链球菌(GAS)是一种病原体,通常通过呼吸道飞沫和皮肤接触传播,估计每年在全球造成 7 亿例轻度非侵入性感染。约有 65 万例感染发展为严重的侵入性感染,甚至导致死亡。因此,快速、准确和实时检测 GAS 的能力非常重要。在此,我们开发了一种纳米酶链接多阵列气体驱动传感器(NLMAGS),可在 2 小时内对 GAS 进行床旁检测。NLMAGS 结合了纳米酶技术、免疫测定技术和 3D 打印技术的优势,表现出卓越的性能。合成了富含铂和钯的纳米酶颗粒(Au@Pt@PdNPs),用于标记单克隆抗体作为检测探针。用单克隆抗体标记磁珠作为捕获探针,建立了一种检测 GAS 的双抗体夹心免疫分析法。夹心免疫复合物可催化 H2O2 底物并产生 O2。通过测量 O2 在传感器中推动墨滴前进的距离,可以实现 GAS 定量。在优化条件下,NLMAGS 可定量检测 24 种添加样品,检测限(LOD)为 62 CFU mL-1,比 ELISA(334 CFU mL-1)低 5 倍。该方法与传统的酶联免疫吸附法有很强的相关性(r = 0.99,P < 0.001)。相比之下,基于 Au@Pt@PdNPs-mAb2 的传统侧流免疫测定(Au@Pt@PdNPs-LFIA)的检测限为 104 CFU mL-1,明显高于 NLMAGS。NLMAGS 对 GAS 的灵敏度极高。传感器的测定内和测定间精度均低于 15%。总之,已建立的 NLMAGS 作为一种快速定量检测 GAS 的方法具有广阔的前景,也可用于检测各种病原体。
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来源期刊
Analyst
Analyst 化学-分析化学
CiteScore
7.80
自引率
4.80%
发文量
636
审稿时长
1.9 months
期刊介绍: The home of premier fundamental discoveries, inventions and applications in the analytical and bioanalytical sciences
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