Formulation and characterization of tea tree and jojoba oils nano-emulgel for in-vivo wound healing assessment

IF 5.4 2区医学 Q1 BIOPHYSICS Colloids and Surfaces B: Biointerfaces Pub Date : 2024-10-09 DOI:10.1016/j.colsurfb.2024.114312

Nehal Kh. Mohamed , Asmaa A. Metwally , Sally M.Y. Fared , A. Farid , Mohamed Taha

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Abstract

Cutaneous wounds are the most common surgical affections among living organisms worldwide, and their healing may be interrupted by several factors. This study aimed to formulate and evaluate the antioxidant, anti-inflammatory, and antimicrobial activity of tea tree and jojoba oils nano-emulsions, additionally, investigating the cytotoxicity of the optimized formula was investigated on normal human lung fibroblast cells (WI-38) by MTT colorimetric assay, additionally its in-vivo wound healing. Nano-emulsions (NEs) were prepared using a high-energy method and characterized by Transmission electron microscopy (TEM), Zeta potential, droplet size, and poly dispersive index (PDI). Nano-emulgel (NEG) was formulated by mixing the standard NE with carbopol® 940. For in-vivo wound healing, thirty adult female albino rats were assigned into control, moist exposed burn ointment (Mebo), and NEG-treated groups. The healing was assessed by analysis of superoxide dismutase (SOD), malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), and histopathology in healed wound tissues. All formulations demonstrated antioxidant, anti-inflammatory, and antimicrobial activity against Bacillus Subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Enterococcus faecalis, and Candida albicans. The CC₅₀ of the optimized formula was 453.82± 3.87 µg/mL, with a mean droplet size of 105.4 nm and a zeta potential of −39.2 ± 2.1 mV. NEG enhanced wound closure compared to Mebo-treated and control groups. Also, MDA significantly decreased and SOD significantly increased in NEG and Mebo-treated groups compared to the control (p ˂ 0.05). TNF-α, and IL-1β significantly decreased in NEG and Mebo-treated compared to the control (p < 0.05). Histopathology revealed reduced inflammatory cell infiltration, rapid epithelization, and increased collagen deposition in NEG-treated wound tissues compared to the control and Mebo-treated wounds. In conclusion, the NEG containing tea tree and jojoba oils demonstrated significant antioxidant, anti-inflammatory, antimicrobial, and wound healing activities.

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用于体内伤口愈合评估的茶树油和荷荷巴油纳米凝胶的配制和表征

皮肤伤口是全球生物体中最常见的外科创伤，其愈合可能会受到多种因素的影响。本研究旨在配制和评估茶树油和荷荷巴油纳米乳液的抗氧化、抗炎和抗菌活性，此外还通过 MTT 比色法研究了优化配方对正常人肺成纤维细胞（WI-38）的细胞毒性，以及其体内伤口愈合情况。采用高能量法制备了纳米乳液（NEs），并通过透射电子显微镜（TEM）、Zeta 电位、液滴大小和聚分散指数（PDI）对其进行了表征。将标准 NE 与 carbopol® 940 混合配制成纳米凝胶（NEG）。为了进行体内伤口愈合，将 30 只成年雌性白化大鼠分为对照组、湿润暴露烧伤膏（Mebo）组和 NEG 处理组。通过分析愈合伤口组织中的超氧化物歧化酶（SOD）、丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和组织病理学来评估伤口愈合情况。所有配方都对枯草杆菌、金黄色葡萄球菌、大肠埃希菌、肺炎克雷伯菌、伤寒沙门氏菌、粪肠球菌和白色念珠菌具有抗氧化、抗炎和抗菌活性。优化配方的 CC50 为 453.82± 3.87 µg/mL，平均液滴大小为 105.4 nm，zeta 电位为 -39.2 ± 2.1 mV。与 Mebo 处理组和对照组相比，NEG 可促进伤口闭合。此外，与对照组相比，NEG 和 Mebo 处理组的 MDA 明显降低，SOD 明显升高（p ˂ 0.05）。与对照组相比，NEG 和 Mebo 处理组的 TNF-α 和 IL-1β 明显降低（p ˂0.05）。组织病理学显示，与对照组和Mebo处理的伤口相比，NEG处理的伤口组织炎症细胞浸润减少，上皮快速形成，胶原沉积增加。总之，含有茶树油和荷荷巴油的 NEG 具有显著的抗氧化、抗炎、抗菌和伤口愈合活性。

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.