Low toxicity ginsenoside Rg1-carbon nanodots as a potential therapeutic agent for human non-small cell lung cancer.

IF 5.4 2区医学 Q1 BIOPHYSICS Colloids and Surfaces B: Biointerfaces Pub Date : 2024-11-20 DOI:10.1016/j.colsurfb.2024.114392

Jifeng Wang, Ning Tian, Tenghui Tian, Lizhi Xiao, Xuechun Zhou, Guancheng Liu, Zhe Zhang, Yu Zhao, Jiajuan Guo, Quan Lin, Yingnan Jiang

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Abstract

Here ginsenoside Rg1 was used to synthesise Rg1 carbon nanodots via a one-step hydrothermal method. The surface of the Rg1 carbon nanodots is rich in hydrophilic functional groups with good water solubility and biocompatibility. The Rg1 carbon nanodots exhibited a high inhibitory effect on the proliferation, migration, and proapoptotic ability of non-small cell lung cancer A549 cells. The changes in the levels of ROS, Ca²⁺, and MMP in A549 cells after the administration of Rg1 carbon nanodots were evaluated and further correlated with relevant proteins in the caspase apoptotic pathway. Proteomic screening revealed that the Rg1 carbon nanodots could regulate A549 cell apoptosis by activating the expression of MAPK pathway-related proteins. In the in vivo experiment, the therapeutic efficacy of the Rg1 carbon nanodots in inhibiting tumour growth was much higher than that of commonly used chemotherapy drugs, with negligible toxicity and side effects. Immunohistochemical staining showed that the expression of caspase- and MAPK pathway-related proteins in mouse tumour tissues was consistent with that at the cellular level. The results suggest that Rg1 carbon nanodots can promote tumour apoptosis and represent a potential therapeutic agent for human non-small-cell lung cancer.

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低毒性人参皂苷 Rg1-碳纳米点作为人类非小细胞肺癌的潜在治疗剂。

本文利用人参皂苷 Rg1 通过一步水热法合成 Rg1 纳米碳点。Rg1 纳米碳点表面富含亲水官能团，具有良好的水溶性和生物相容性。Rg1 纳米碳点对非小细胞肺癌 A549 细胞的增殖、迁移和促凋亡能力具有很强的抑制作用。研究还评估了 Rg1 碳纳米点施用后 A549 细胞中 ROS、Ca2+ 和 MMP 水平的变化，并进一步将其与 Caspase 细胞凋亡通路中的相关蛋白进行了关联。蛋白质组学筛选显示，Rg1 纳米碳点能通过激活 MAPK 通路相关蛋白的表达来调节 A549 细胞的凋亡。在体内实验中，Rg1 碳纳米点抑制肿瘤生长的疗效远高于常用化疗药物，且毒副作用微乎其微。免疫组化染色显示，小鼠肿瘤组织中的 Caspase- 和 MAPK 通路相关蛋白的表达与细胞水平一致。结果表明，Rg1 碳纳米点可促进肿瘤细胞凋亡，是一种潜在的人类非小细胞肺癌治疗药物。

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.