A novel label-free electrochemical immunosensor based on Ce-MOF@AgAuNPs for highly sensitive detection of monensin

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Food Control Pub Date : 2024-10-02 DOI:10.1016/j.foodcont.2024.110927

Yiting Zhou , Jingming Zhou , Yankai Liu , Yumei Chen , Hongliang Liu , Xifang Zhu , Peiyang Ding , Chao Liang , Enping Liu , Sixuan Wu , Aiping Wang

{"title":"A novel label-free electrochemical immunosensor based on Ce-MOF@AgAuNPs for highly sensitive detection of monensin","authors":"Yiting Zhou , Jingming Zhou , Yankai Liu , Yumei Chen , Hongliang Liu , Xifang Zhu , Peiyang Ding , Chao Liang , Enping Liu , Sixuan Wu , Aiping Wang","doi":"10.1016/j.foodcont.2024.110927","DOIUrl":null,"url":null,"abstract":"<div><div>A novel electrochemical immunosensor for the sensitive detection of monensin (MON) was developed. The sensor platform utilized anti-MON monoclonal antibodies and a nanocomposite (Ce-MOF@AgAuNPs), composed of cerium-based metal-organic frameworks decorated with silver-gold nanoparticles. The physicochemical properties of the Ce-MOF@AgAuNPs nanocomposite were investigated using transmission electron microscopy, energy dispersive X-ray, UV–Vis spectrophotometry, and fourier transform infrared techniques. The stepwise fabrication process and electrochemical performance of the immunosensor were investigated through electrochemical impedance spectroscopy and cyclic voltammetry. The sensor exhibited excellent analytical performance for MON detection, with a wide linear response range from 0.05 to 250 ng mL<sup>−1</sup> and a low LOD of 0.008 ng mL<sup>−1</sup>. Additionally, the immunosensor exhibited high accuracy, with recovery rates ranging from 97.4% to 103.2% in chicken liver and 96.0%–104.7% in milk. This developed immunosensor shows great potential for food safety applications, demonstrating high specificity, stability, and reproducibility in the detection of MON.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"168 ","pages":"Article 110927"},"PeriodicalIF":5.6000,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Control","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956713524006443","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

A novel electrochemical immunosensor for the sensitive detection of monensin (MON) was developed. The sensor platform utilized anti-MON monoclonal antibodies and a nanocomposite (Ce-MOF@AgAuNPs), composed of cerium-based metal-organic frameworks decorated with silver-gold nanoparticles. The physicochemical properties of the Ce-MOF@AgAuNPs nanocomposite were investigated using transmission electron microscopy, energy dispersive X-ray, UV–Vis spectrophotometry, and fourier transform infrared techniques. The stepwise fabrication process and electrochemical performance of the immunosensor were investigated through electrochemical impedance spectroscopy and cyclic voltammetry. The sensor exhibited excellent analytical performance for MON detection, with a wide linear response range from 0.05 to 250 ng mL⁻¹ and a low LOD of 0.008 ng mL⁻¹. Additionally, the immunosensor exhibited high accuracy, with recovery rates ranging from 97.4% to 103.2% in chicken liver and 96.0%–104.7% in milk. This developed immunosensor shows great potential for food safety applications, demonstrating high specificity, stability, and reproducibility in the detection of MON.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

基于 Ce-MOF@AgAuNPs 的新型无标记电化学免疫传感器用于高灵敏度检测莫能菌素

本研究开发了一种用于灵敏检测莫能菌素（MON）的新型电化学免疫传感器。该传感器平台利用了抗 MON 单克隆抗体和一种纳米复合材料（Ce-MOF@AgAuNPs），后者由铈基金属有机框架和银金纳米粒子装饰组成。利用透射电子显微镜、能量色散 X 射线、紫外可见分光光度法和傅立叶变换红外技术研究了 Ce-MOF@AgAuNPs 纳米复合材料的理化性质。通过电化学阻抗谱和循环伏安法研究了免疫传感器的分步制备过程和电化学性能。该传感器在检测 MON 方面表现出优异的分析性能，线性响应范围从 0.05 到 250 ng mL-1 不等，LOD 低至 0.008 ng mL-1。此外，该免疫传感器的准确度也很高，在鸡肝中的回收率为 97.4% 至 103.2%，在牛奶中的回收率为 96.0% 至 104.7%。所开发的这种免疫传感器在检测 MON 方面具有高特异性、稳定性和可重复性，在食品安全应用方面显示出巨大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Food Control 工程技术-食品科技

CiteScore

12.20

自引率

6.70%

发文量

758

审稿时长

33 days

期刊介绍： Food Control is an international journal that provides essential information for those involved in food safety and process control. Food Control covers the below areas that relate to food process control or to food safety of human foods: • Microbial food safety and antimicrobial systems • Mycotoxins • Hazard analysis, HACCP and food safety objectives • Risk assessment, including microbial and chemical hazards • Quality assurance • Good manufacturing practices • Food process systems design and control • Food Packaging technology and materials in contact with foods • Rapid methods of analysis and detection, including sensor technology • Codes of practice, legislation and international harmonization • Consumer issues • Education, training and research needs. The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.