Jing Mo , Shiqi Yu , Zhaohua Shi , Hailan Zhu , Lichun Ye , Yuxin Zhou , Jing Nie , Bo Wang
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引用次数: 0
Abstract
Aesculus wilsonii Rehd, a medicinal food homology plant, whose fruit are used to treat diseases such as edema and exudation of soft tissue. Nonetheless, the presence of microbial and mycotoxin contaminants in A. wilsonii fruit is still unclear. In this study, high-throughput sequencing and liquid chromatograph tandem-mass spectrometry were conducted to determine the diversity of fungal microbiome and mycotoxin contents in moldy A. wilsonii fruit. The prevalent fungi in A. wilsonii fruit included Fusarium, Colletotrichum, Dictyostelium, and Aspergillus. Furthermore, mycotoxin tests showed that all samples tested positive for ochratoxin A, with deoxynivalenol detected in 72.4 % of samples, zearalenone in 24.1 %, and T-2 toxin in 62.1 %, revealing these mycotoxins constituted the primary contaminants. Moreover, to monitor the mycotoxin-producing fungi, multiplex ligation-dependent probe amplification (MLPA) targeting key genes in toxin biosynthetic pathway of Fusarium and Aspergillus was established. and it can detect fungi with DNA content less than 20 ng. In conclusion, this study reported the microbiome diversity and the prevalence of fungi contamination in A. wilsonii fruit, while also established a rapid method for detecting toxigenic fungi. This work focused on analyzing and detecting microbiomes and mycotoxins in A. wilsonii fruit, providing a reference for the study of potentially harmful microorganisms in the storage of medicinal products.
期刊介绍:
JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.