Evaluation of size-dependent uptake, transport and cytotoxicity of polystyrene microplastic in a blood-brain barrier (BBB) model

IF 13.4 2区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY Nano Convergence Pub Date : 2024-10-15 DOI:10.1186/s40580-024-00448-z
Yeongseon Cho, Eun U Seo, Kyeong Seob Hwang, Hyelim Kim, Jonghoon Choi, Hong Nam Kim
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Abstract

Microplastics, particularly those in the micrometer scale, have been shown to enter the human body through ingestion, inhalation, and dermal contact. Recent research indicates that microplastics can potentially impact the central nervous system (CNS) by crossing the blood-brain barrier (BBB). However, the exact mechanisms of their transport, uptake, and subsequent toxicity at BBB remain unclear. In this study, we evaluated the size-dependent uptake and cytotoxicity of polystyrene microparticles using an engineered BBB model. Our findings demonstrate that 0.2 μm polystyrene microparticles exhibit significantly higher uptake and transendothelial transport compared to 1.0 μm polystyrene microparticles, leading to increased permeability and cellular damage. After 24 h of exposure, permeability increased by 15.6-fold for the 0.2 μm particles and 2-fold for the 1.0 μm particles compared to the control. After 72 h of exposure, permeability further increased by 27.3-fold for the 0.2 μm particles and a 4.5-fold for the 1.0 μm particles compared to the control. Notably, microplastics administration following TNF-α treatment resulted in enhanced absorption and greater BBB damage compared to non-stimulated conditions. Additionally, the size-dependent toxicity observed differently between 2D cultured cells and 3D BBB models, highlighting the importance of testing models in evaluating environmental toxicity.

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在血脑屏障(BBB)模型中评估聚苯乙烯微塑料随尺寸变化的吸收、运输和细胞毒性
研究表明,微塑料,尤其是微米级的微塑料,可通过摄入、吸入和皮肤接触进入人体。最新研究表明,微塑料有可能通过穿过血脑屏障(BBB)影响中枢神经系统(CNS)。然而,微塑料在 BBB 转运、吸收和随后产生毒性的确切机制仍不清楚。在这项研究中,我们利用工程化 BBB 模型评估了聚苯乙烯微颗粒的大小依赖性吸收和细胞毒性。我们的研究结果表明,与 1.0 微米的聚苯乙烯微颗粒相比,0.2 微米的聚苯乙烯微颗粒表现出明显更高的吸收和跨内皮转运能力,从而导致通透性增加和细胞损伤。与对照组相比,接触 24 小时后,0.2 μm 粒子的渗透性增加了 15.6 倍,1.0 μm 粒子的渗透性增加了 2 倍。接触 72 小时后,与对照组相比,0.2 μm 粒子的渗透性进一步增加了 27.3 倍,1.0 μm 粒子的渗透性增加了 4.5 倍。值得注意的是,与未受刺激的情况相比,在TNF-α处理后施用微塑料会促进吸收并加重BBB损伤。此外,在二维培养细胞和三维 BBB 模型中观察到的尺寸依赖性毒性也不同,这突出了在评估环境毒性时测试模型的重要性。
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来源期刊
Nano Convergence
Nano Convergence Engineering-General Engineering
CiteScore
15.90
自引率
2.60%
发文量
50
审稿时长
13 weeks
期刊介绍: Nano Convergence is an internationally recognized, peer-reviewed, and interdisciplinary journal designed to foster effective communication among scientists spanning diverse research areas closely aligned with nanoscience and nanotechnology. Dedicated to encouraging the convergence of technologies across the nano- to microscopic scale, the journal aims to unveil novel scientific domains and cultivate fresh research prospects. Operating on a single-blind peer-review system, Nano Convergence ensures transparency in the review process, with reviewers cognizant of authors' names and affiliations while maintaining anonymity in the feedback provided to authors.
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