Transmission of carbapenem-resistant enterobacterales producing NDM-5 during the broiler breeding process in China

IF 2.4 2区农林科学 Q3 MICROBIOLOGY Veterinary microbiology Pub Date : 2024-10-16 DOI:10.1016/j.vetmic.2024.110282

Ke Wu , Yuanyuan Zhang , Wei Xu , Xiaolong Lin , Cui Li , Juan Wang , Ruichao Li , Yizhi Tang , Changwei Lei , Hongning Wang

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Abstract

This study conducted a four-month monitoring of carbapenem resistance in a broiler breeding farm in China. A total of 185 carbapenem-resistant bacterial isolates were obtained from 2298 cloacal swabs from broiler breeders and their offspring within a production cycle. The detection rate of carbapenem-resistant isolates was higher during the brooding period. Whole-genome sequencing (WGS) was performed on 133 isolates based on sampling stages, including 113 carbapenem-resistant Enterobacterales (CRE) isolates and 20 Stenotrophomonas pavanii isolates, which have intrinsic resistance to carbapenems. A total of 69 antibiotic resistance genes (ARGs), including bla_NDM-1, mcr-1, and bla_NDM-5, were identified among the sequenced CRE isolates. Notably, bla_NDM-5 (92.0 %, 104/113) was the primary contributor to carbapenem resistance. CRE isolates from the same breeding stage exhibited close genomic relationships, and the bla_NDM-5 genes were observed in similar genetic backgrounds, indicating the transmission of CRE strains and bla_NDM-5 during the broiler breeding process. No CRE was isolated from 0 d broiler offspring, suggesting that broiler breeders were not the direct source of CRE in their offspring. Tracing the feeding process revealed that brooder and rearing houses were likely key factors in the cross-transmission of CRE between broiler breeders and their offspring. CRE pose a significant threat to public health and food safety. China is one of the world's leading poultry producing and consuming countries. This study provided insights into the epidemiological trends and key transmission nodes of carbapenem resistance and CRE within the broiler breeding process, which could help the control of antibiotic resistance and bacterial infections in the broiler industry.

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中国肉鸡养殖过程中产生NDM-5的耐碳青霉烯类肠杆菌的传播

本研究对中国一家肉种鸡养殖场的碳青霉烯类耐药性进行了为期四个月的监测。在一个生产周期内，从肉用种鸡及其后代的 2298 份泄殖腔拭子中共分离出 185 株耐碳青霉烯类细菌。在育雏期，耐碳青霉烯类细菌分离株的检出率较高。根据采样阶段，对 133 个分离物进行了全基因组测序（WGS），其中包括 113 个耐碳青霉烯类肠杆菌（CRE）分离物和 20 个对碳青霉烯类有内在耐药性的铺满性苯并酵母菌（Stenotrophomonas pavanii）分离物。在测序的 CRE 分离物中共鉴定出 69 个抗生素耐药基因（ARGs），包括 blaNDM-1、mcr-1 和 blaNDM-5。值得注意的是，blaNDM-5（92.0%，104/113）是造成碳青霉烯类耐药性的主要原因。来自同一育种阶段的 CRE 分离物表现出密切的基因组关系，并且在相似的遗传背景中观察到 blaNDM-5 基因，这表明 CRE 菌株和 blaNDM-5 在肉鸡育种过程中存在传播。从 0 d 后代肉鸡中未分离到 CRE，表明肉种鸡不是后代中 CRE 的直接来源。对饲养过程的追踪表明，育雏器和育雏舍可能是肉种鸡及其后代之间交叉传播 CRE 的关键因素。CRE对公众健康和食品安全构成重大威胁。中国是世界上主要的家禽生产国和消费国之一。本研究深入探讨了肉鸡养殖过程中碳青霉烯类耐药性和CRE的流行趋势和关键传播节点，有助于控制肉鸡行业的抗生素耐药性和细菌感染。

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.