{"title":"A Planar Darkfield Illuminator for Wide‐Field High‐Contrast Imaging of Specimens in Microfluidic Chips","authors":"Zetao Fan, Xiao Yang, Xinxiang You, Ke Yang, Ling Zhu, Douguo Zhang","doi":"10.1002/lpor.202400573","DOIUrl":null,"url":null,"abstract":"Darkfield microscopy is an effective technique and well suited for use involving live and unstained biological samples, where a bulky condenser should be precisely aligned in the optical path to produce a cone of illumination light. Here, a compact darkfield illuminator is invented that has a planar shape and can work at broadband wavelengths. It can be easily incorporated into a conventional microscope as a substrate to produce hollow cones of light for large‐area darkfield illumination, resulting in a high‐contrast high‐sensitivity widefield microscopy images. Taking advantages of its planar and compact structure, this darkfield illuminator is successfully integrated with a microfluidic chip; thus, the actions and reactions of biological and chemical specimens flowing inside micrometer‐sized channels, can be real‐time imaged with high contrast and sensitivity in a large field‐of‐view (FoV). For the first time, the work integrates a compact darkfield illuminator with microfluidic chips, which produces a new platform for applications in biology, chemistry, and biomedical engineering.","PeriodicalId":204,"journal":{"name":"Laser & Photonics Reviews","volume":null,"pages":null},"PeriodicalIF":9.8000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Laser & Photonics Reviews","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1002/lpor.202400573","RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 0
Abstract
Darkfield microscopy is an effective technique and well suited for use involving live and unstained biological samples, where a bulky condenser should be precisely aligned in the optical path to produce a cone of illumination light. Here, a compact darkfield illuminator is invented that has a planar shape and can work at broadband wavelengths. It can be easily incorporated into a conventional microscope as a substrate to produce hollow cones of light for large‐area darkfield illumination, resulting in a high‐contrast high‐sensitivity widefield microscopy images. Taking advantages of its planar and compact structure, this darkfield illuminator is successfully integrated with a microfluidic chip; thus, the actions and reactions of biological and chemical specimens flowing inside micrometer‐sized channels, can be real‐time imaged with high contrast and sensitivity in a large field‐of‐view (FoV). For the first time, the work integrates a compact darkfield illuminator with microfluidic chips, which produces a new platform for applications in biology, chemistry, and biomedical engineering.
期刊介绍:
Laser & Photonics Reviews is a reputable journal that publishes high-quality Reviews, original Research Articles, and Perspectives in the field of photonics and optics. It covers both theoretical and experimental aspects, including recent groundbreaking research, specific advancements, and innovative applications.
As evidence of its impact and recognition, Laser & Photonics Reviews boasts a remarkable 2022 Impact Factor of 11.0, according to the Journal Citation Reports from Clarivate Analytics (2023). Moreover, it holds impressive rankings in the InCites Journal Citation Reports: in 2021, it was ranked 6th out of 101 in the field of Optics, 15th out of 161 in Applied Physics, and 12th out of 69 in Condensed Matter Physics.
The journal uses the ISSN numbers 1863-8880 for print and 1863-8899 for online publications.