Structural insights into the membrane-bound proteolytic machinery of bacterial protein quality control.

IF 3.8 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical Society transactions Pub Date : 2024-10-30 DOI:10.1042/BST20231250
Rya Ero, Zhu Qiao, Kwan Ann Tan, Yong-Gui Gao
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Abstract

In bacteria and eukaryotic organelles of prokaryotic origin, ATP-dependent proteases are crucial for regulating protein quality control through substrate unfolding and degradation. Understanding the mechanism and regulation of this key cellular process could prove instrumental in developing therapeutic strategies. Very recently, cryo-electron microscopy structural studies have shed light on the functioning of AAA+ proteases, including membrane-bound proteolytic complexes. This review summarizes the structure and function relationship of bacterial AAA+ proteases, with a special focus on the sole membrane-bound AAA+ protease in Escherichia coli, FtsH. FtsH substrates include both soluble cytoplasmic and membrane-incorporated proteins, highlighting its intricate substrate recognition and processing mechanisms. Notably, 12 copies of regulatory HflK and HflC proteins, arranged in a cage-like structure embedded in the bacterial inner membrane, can encase up to 4 FtsH hexamers, thereby regulating their role in membrane protein quality control. FtsH represents an intriguing example, highlighting both its similarity to cytosolic AAA+ proteases with respect to overall architecture and oligomerization as well as its unique features, foremost its incorporation into a membrane-bound complex formed by HflK and HflC to mediate its function in protein quality control.

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从结构上洞察细菌蛋白质质量控制的膜结合蛋白水解机制。
在细菌和原核生物的真核细胞器中,依赖 ATP 的蛋白酶对通过底物展开和降解来调节蛋白质质量控制至关重要。了解这一关键细胞过程的机制和调控方法有助于制定治疗策略。最近,冷冻电镜结构研究揭示了 AAA+蛋白酶的功能,包括膜结合蛋白水解复合物。本综述总结了细菌 AAA+ 蛋白酶的结构与功能关系,并特别关注大肠杆菌中唯一的膜结合型 AAA+ 蛋白酶 FtsH。FtsH 的底物包括可溶性细胞质蛋白和入膜蛋白,突出了其复杂的底物识别和处理机制。值得注意的是,12 个拷贝的调节性 HflK 和 HflC 蛋白排列在一个嵌入细菌内膜的笼状结构中,可包裹多达 4 个 FtsH 六聚体,从而调节它们在膜蛋白质量控制中的作用。FtsH 是一个耐人寻味的例子,既突出了它在整体结构和寡聚化方面与细胞质 AAA+ 蛋白酶的相似性,也突出了它的独特性,最重要的是它被纳入了由 HflK 和 HflC 形成的膜结合复合物,从而介导了它在蛋白质质量控制方面的功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biochemical Society transactions
Biochemical Society transactions 生物-生化与分子生物学
CiteScore
7.80
自引率
0.00%
发文量
351
审稿时长
3-6 weeks
期刊介绍: Biochemical Society Transactions is the reviews journal of the Biochemical Society. Publishing concise reviews written by experts in the field, providing a timely snapshot of the latest developments across all areas of the molecular and cellular biosciences. Elevating our authors’ ideas and expertise, each review includes a perspectives section where authors offer comment on the latest advances, a glimpse of future challenges and highlighting the importance of associated research areas in far broader contexts.
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