Isolation and evaluation of the pathogenicity of a hybrid shiga toxin-producing and Enterotoxigenic Escherichia coli in pigs.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES BMC Veterinary Research Pub Date : 2024-10-21 DOI:10.1186/s12917-024-04317-z
Danaya Nammuang, Yi-Wen Shen, Chiao-Hsu Ke, Nan-Ling Kuan, Chao-Nan Lin, Kuang-Sheng Yeh, Yen-Chen Chang, Chia-Yu Chang, Hui-Wen Chang
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Abstract

Background: Porcine pathogenic Escherichia coli (E. coli), the globally recognized important pathogen, causes significant economic loss in the field. Enterotoxigenic E. coli (ETEC) causes porcine neonatal and post-weaning diarrhea (PWD), frequently carrying F4 adhesin, F18 adhesin, Heat-Stable toxin (ST), and Heat-Labile toxin (LT). Shiga Toxin-Producing E. coli (STEC) produces F18 adhesin and Shiga toxin type 2e (stx2e), majorly leading to systemic endothelial cell damage and edema disease. In this study, hemolytic pathogenic hybrid STEC/ETEC strains carrying ST and LT genes of ETEC and the Stx2e gene of STEC isolated from pigs with PWD in Taiwan were identified. The pathogenicity of a Taiwan hybrid STEC/ETEC strain was evaluated by oral inoculation in post-weaning pigs.

Results: Next generation sequencing and multilocus sequence typing of two hybrid Taiwan porcine STEC/ETEC isolates indicated that these two isolates were closely related to the ST88 porcine hybrid STEC/ETEC isolated from pigs with watery diarrhea. Furthermore, the two hybrid Taiwan porcine STEC/ETEC isolates also displayed combinations of multiple resistance genes encoding mechanisms for target modification and antibiotic inactivation. Animal experiments confirmed that the Taiwan hybrid STEC/ETEC could cause watery diarrhea in post-weaning pigs with no signs of edema disease and minimal histopathological lesions.

Conclusion: To the best of the authors' knowledge, the present study is the first study demonstrating intestinal pathogenicity of the hybrid STEC/ETEC in pigs. The result suggests that the hybrid STEC/ETEC should be considered as a new emerging pathogen and a new target for vaccine development.

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猪中产志贺毒素和肠毒性大肠杆菌杂交种的分离和致病性评估。
背景:猪致病性大肠杆菌(E. coli)是全球公认的重要病原体,在田间造成重大经济损失。肠毒性大肠杆菌(ETEC)会导致猪新生儿和断奶后腹泻(PWD),经常携带 F4 粘附素、F18 粘附素、热稳定毒素(ST)和热凋亡毒素(LT)。产志贺毒素大肠杆菌(STEC)产生 F18 粘附素和志贺毒素 2e 型(stx2e),主要导致全身内皮细胞损伤和水肿病。本研究鉴定了从台湾患有脊髓灰质炎的猪只中分离出的携带 ETEC 的 ST 和 LT 基因以及 STEC 的 Stx2e 基因的溶血性致病性 STEC/ETEC 杂交菌株。通过对断奶后的猪进行口服接种,评估了台湾杂交 STEC/ETEC 株系的致病性:结果:对两株台湾杂交猪 STEC/ETEC 分离物进行的新一代测序和多焦点序列分型表明,这两株分离物与从水泻猪体内分离出的 ST88 猪杂交 STEC/ETEC 关系密切。此外,这两种台湾杂交猪 STEC/ETEC 分离物还显示出多种抗性基因的组合,编码靶向修饰和抗生素失活机制。动物实验证实,台湾杂交猪 STEC/ETEC 可引起断奶后猪水样腹泻,且无水肿病症状,组织病理学病变极小:据作者所知,本研究是首次证明猪杂交 STEC/ETEC 肠道致病性的研究。结果表明,杂交 STEC/ETEC 应被视为一种新出现的病原体和疫苗开发的新目标。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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