Screening of antimicrobial activity in venom: Exploring key parameters

IF 2.6 4区 医学 Q2 PHARMACOLOGY & PHARMACY Toxicon Pub Date : 2024-10-20 DOI:10.1016/j.toxicon.2024.108135
Awatef Ouertani , Chloé Mollet , Yasmine Boughanmi , Harold de Pomyers , Amor Mosbah , Hadda-Imene Ouzari , Ameur Cherif , Didier Gigmes , Marc Maresca , Kamel Mabrouk
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Abstract

The escalating challenge of antibiotic resistance significantly threatens global health, underscoring the critical need for new antimicrobial agents. Venoms, increasingly recognized as reservoirs of bioactive compounds with diverse pharmacological effects, have been the focus of recent research. This work evaluates the use of various screening methodologies in assessing the antimicrobial activities of 185 venoms against some gram positive and gram negative bacteria, including E. coli ATCC 8739, B. subtilis ATCC 6633, P. aeruginosa ATCC 9027, and S. aureus ATCC 6538P species and explores the influence of settings on the findings. Furthermore, the research explored the possibility of purifying antimicrobial molecules from venoms through HPLC. Several fractions demonstrated antimicrobial activity against the tested strains.
Our results reveal that the measured antimicrobial efficacy of venoms varies according to:i) venom concentration, ii) the detection method, including microdilution and radial diffusion assays, and iii) the choice of culture medium, specifically LB or MH.
This strategy has allowed us, for the first time, to identify antimicrobial activity in: i) Bitis arietans venom against P. aeruginosa ATCC 9027, ii) Naja nubiae and Bothrops lanceolatus against B. subtilis ATCC 6633, P. aeruginosa ATCC 9027, and S. aureus ATCC 6538P, and iii) Hadogenes zuluanus, Mesobuthus caucasicus, Nebo hierichonticus, Opistophthalmus wahlbergii scorpions, and Mylabris quadripunctata beetles against S. aureus ATCC 6538P.
These findings highlight venoms potential as effective antimicrobial resources and improve our understanding of key factors critical for an accurate detection of venoms antimicrobial properties.

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筛选毒液中的抗菌活性:探索关键参数。
抗生素耐药性的不断升级严重威胁着全球健康,这突出表明了对新型抗菌剂的迫切需要。毒液越来越被认为是具有多种药理作用的生物活性化合物的宝库,是近期研究的重点。这项工作评估了使用各种筛选方法评估 185 种毒液对一些革兰氏阳性和革兰氏阴性细菌的抗菌活性,包括大肠杆菌 ATCC 8739、枯草杆菌 ATCC 6633、铜绿假单胞菌 ATCC 9027 和金黄色葡萄球菌 ATCC 6538P,并探讨了环境对研究结果的影响。此外,研究还探讨了通过高效液相色谱法从毒液中纯化抗菌分子的可能性。一些馏分对测试菌株具有抗菌活性。我们的研究结果表明,毒液的抗菌效力因以下因素而异:(i) 毒液浓度;(ii) 检测方法,包括微量稀释法和径向扩散法;(iii) 培养基的选择,特别是 LB 或 MH。通过这一策略,我们首次确定了以下几种毒液的抗菌活性:i) Bitis arietans 毒液对铜绿假单胞菌 ATCC 9027 的抗菌活性;ii) Naja nubiae 和 Bothrops lanceolatus 毒液对枯草杆菌 ATCC 6633、铜绿假单胞菌 ATCC 9027 和枯草杆菌 ATCC 6633 的抗菌活性。铜绿假单胞菌 ATCC 9027 和金黄色葡萄球菌 ATCC 6538P;以及 iii) Hadogenes zuluanus、Mesobuthus caucasicus、Nebo hierichonticus、Opistophthalmus wahlbergii 蝎子和 Mylabris quadripunctata 甲虫对金黄色葡萄球菌 ATCC 6538P。这些发现凸显了毒液作为有效抗菌资源的潜力,并增进了我们对准确检测毒液抗菌特性关键因素的了解。
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来源期刊
Toxicon
Toxicon 医学-毒理学
CiteScore
4.80
自引率
10.70%
发文量
358
审稿时长
68 days
期刊介绍: Toxicon has an open access mirror Toxicon: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. An introductory offer Toxicon: X - full waiver of the Open Access fee. Toxicon''s "aims and scope" are to publish: -articles containing the results of original research on problems related to toxins derived from animals, plants and microorganisms -papers on novel findings related to the chemical, pharmacological, toxicological, and immunological properties of natural toxins -molecular biological studies of toxins and other genes from poisonous and venomous organisms that advance understanding of the role or function of toxins -clinical observations on poisoning and envenoming where a new therapeutic principle has been proposed or a decidedly superior clinical result has been obtained. -material on the use of toxins as tools in studying biological processes and material on subjects related to venom and antivenom problems. -articles on the translational application of toxins, for example as drugs and insecticides -epidemiological studies on envenoming or poisoning, so long as they highlight a previously unrecognised medical problem or provide insight into the prevention or medical treatment of envenoming or poisoning. Retrospective surveys of hospital records, especially those lacking species identification, will not be considered for publication. Properly designed prospective community-based surveys are strongly encouraged. -articles describing well-known activities of venoms, such as antibacterial, anticancer, and analgesic activities of arachnid venoms, without any attempt to define the mechanism of action or purify the active component, will not be considered for publication in Toxicon. -review articles on problems related to toxinology. To encourage the exchange of ideas, sections of the journal may be devoted to Short Communications, Letters to the Editor and activities of the affiliated societies.
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