Behavior and oxidative stress evaluation of scorpion Tityus serrulatus (Lutz & Mello,1922) envenomation with genomic modulation and dopaminergic neutralization by antiscorpionic serum treatment.

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY Toxicon Pub Date : 2025-01-24 DOI:10.1016/j.toxicon.2025.108263

Rahisa Scussel, Mírian Ívens Fagundes, Gabriel Paulino Luiz, Nathalia Coral Galvani, Fernanda F Gava, Ellen De Pieri, Lariani Tamires Witt Tietbohl, Taise Possamai-Della, Jorge M Aguiar-Geraldo, Samira S Valvassori, Vanessa Moraes de Andrade, Carlos Chávez-Olórtegui, Ricardo Andrez Machado-de-Ávila

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引用次数: 0

Abstract

Tityus serrulatus accident promote vast symptomatology related to toxins of the venom, which leads to a massive release of neurotransmitters, notably dopamine, affecting behavior and neurochemistry. The recommended treatment for envenomation is the antiscorpionic serum (SAEsc) administration. Related to this complexity of the Tityus serrulatus envenomation, this study aimed to assess organism responses to the venom, its impact on behavior, oxidative stress, neurochemistry, and genetic impacts, as well as the efficacy of SAEsc, especially concerning dopamine levels and genetic interactions. Swiss mice were divided into groups and administered different venom concentrations intracerebroventricularly to assess behavioral impacts and brain oxidative stress. Oxidative stress was evaluated through reactive oxygen species (ROS) analysis and antioxidant assays, including dichloro-dihydro-fluorescein diacetate (DCF), thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), and glutathione (GSH) measurements. Swiss mice were divided into four groups to evaluate genomic modulation, micronucleus enhancement, and dopamine levels. Additionally, SAEsc's neutralizing effect on dopamine was also investigated. Results showed that venom doses (100-300 ng/μL) increased lipid peroxidation in the brain, with SAEsc maintaining dopamine balance and neutralizing venom up to 24 hours post-envenomation. After 24 hours, cellular repair became less efficient, leading to mutagenic damage in both treated and untreated animals. The results highlight the importance of considering genomic and neurotransmitter function modulation in the treatment of Tityus serrulatus envenomation.

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来源期刊

Toxicon 医学-毒理学

CiteScore

4.80

自引率

10.70%

发文量

358

审稿时长

68 days

期刊介绍： Toxicon has an open access mirror Toxicon: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. An introductory offer Toxicon: X - full waiver of the Open Access fee. Toxicon''s "aims and scope" are to publish: -articles containing the results of original research on problems related to toxins derived from animals, plants and microorganisms -papers on novel findings related to the chemical, pharmacological, toxicological, and immunological properties of natural toxins -molecular biological studies of toxins and other genes from poisonous and venomous organisms that advance understanding of the role or function of toxins -clinical observations on poisoning and envenoming where a new therapeutic principle has been proposed or a decidedly superior clinical result has been obtained. -material on the use of toxins as tools in studying biological processes and material on subjects related to venom and antivenom problems. -articles on the translational application of toxins, for example as drugs and insecticides -epidemiological studies on envenoming or poisoning, so long as they highlight a previously unrecognised medical problem or provide insight into the prevention or medical treatment of envenoming or poisoning. Retrospective surveys of hospital records, especially those lacking species identification, will not be considered for publication. Properly designed prospective community-based surveys are strongly encouraged. -articles describing well-known activities of venoms, such as antibacterial, anticancer, and analgesic activities of arachnid venoms, without any attempt to define the mechanism of action or purify the active component, will not be considered for publication in Toxicon. -review articles on problems related to toxinology. To encourage the exchange of ideas, sections of the journal may be devoted to Short Communications, Letters to the Editor and activities of the affiliated societies.