Comparative Transcriptomic Analyses Reveal Differences in the Responses of Diploid and Triploid Eastern Oysters to Environmental Stress

IF 3.5 2区 生物学 Q1 EVOLUTIONARY BIOLOGY Evolutionary Applications Pub Date : 2024-10-22 DOI:10.1111/eva.70028
Rujuta V. Vaidya, Sarah Bodenstein, Dildorakhon Rasulova, Jerome F. La Peyre, Morgan W. Kelly
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Abstract

Triploid oysters are commonly used as the basis for production in the aquaculture of eastern oysters along the USA East and Gulf of Mexico coasts. While they are valued for their rapid growth, incidents of triploid mortality during summer months have been well documented in eastern oysters, especially at low salinity sites. We compared global transcriptomic responses of diploid and triploid oysters bred from the same three maternal source populations at two different hatcheries and outplanted to a high (annual mean salinity = 19.4 ± 6.7) and low (annual mean salinity = 9.3 ± 5.0) salinity site. Oysters were sampled for gene expression at the onset of a mortality event in the summer of 2021 to identify triploid-specific gene expression patterns associated with low salinity sites, which ultimately experienced greater triploid mortality. We also examined chromosome-specific gene expression to test for instances of aneuploidy in experimental triploid oyster lines, another possible contributor to elevated mortality in triploids. We observed a strong effect of hatchery conditions (cohort) on triploid-specific mortality (field data) and a strong interactive effect of hatchery, ploidy, and outplant site on gene expression. At the low salinity site where triploid oysters experienced high mortality, we observed downregulation of transcripts related to calcium signaling, ciliary activity, and cell cycle checkpoints in triploids relative to diploids. These transcripts suggest dampening of the salinity stress response and problems during cell division as key cellular processes associated with elevated mortality risk in triploid oysters. No instances of aneuploidy were detected in our triploid oyster lines. Our results suggest that triploid oysters may be fundamentally less tolerant of rapid decreases in salinity, indicating that oyster farmers may need to limit the use of triploid oysters to sites with more stable salinity conditions.

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转录组比较分析揭示二倍体和三倍体东方牡蛎对环境压力的反应差异
在美国东部和墨西哥湾沿岸养殖东部牡蛎时,通常使用三倍体牡蛎作为生产基础。虽然三倍体牡蛎因其生长迅速而受到重视,但在夏季,三倍体牡蛎死亡的事件却屡见报端,尤其是在低盐度地区。我们比较了二倍体和三倍体牡蛎的全球转录组反应,二倍体和三倍体牡蛎是在两个不同的孵化场从相同的三个母源种群培育出来的,并分别移植到高盐度(年平均盐度 = 19.4 ± 6.7)和低盐度(年平均盐度 = 9.3 ± 5.0)地区。在 2021 年夏季死亡事件开始时对牡蛎进行基因表达取样,以确定与低盐度地点相关的三倍体特异性基因表达模式,低盐度地点最终经历了更高的三倍体死亡率。我们还研究了染色体特异性基因表达,以检测实验性三倍体牡蛎品系中的非整倍体情况,这可能是导致三倍体死亡率升高的另一个原因。我们观察到孵化条件(群组)对三倍体特异死亡率(现场数据)的强烈影响,以及孵化、倍性和移植地点对基因表达的强烈交互影响。在三倍体牡蛎死亡率较高的低盐度地区,我们观察到与钙信号转导、纤毛活动和细胞周期检查点有关的转录本相对于二倍体有所下调。这些转录本表明,盐度应激反应的抑制和细胞分裂过程中的问题是三倍体牡蛎死亡风险升高的关键细胞过程。在我们的三倍体牡蛎品系中没有发现非整倍体的情况。我们的研究结果表明,三倍体牡蛎对盐度急剧下降的耐受性可能较差,这表明牡蛎养殖者可能需要将三倍体牡蛎的使用限制在盐度条件较为稳定的地点。
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来源期刊
Evolutionary Applications
Evolutionary Applications 生物-进化生物学
CiteScore
8.50
自引率
7.30%
发文量
175
审稿时长
6 months
期刊介绍: Evolutionary Applications is a fully peer reviewed open access journal. It publishes papers that utilize concepts from evolutionary biology to address biological questions of health, social and economic relevance. Papers are expected to employ evolutionary concepts or methods to make contributions to areas such as (but not limited to): medicine, agriculture, forestry, exploitation and management (fisheries and wildlife), aquaculture, conservation biology, environmental sciences (including climate change and invasion biology), microbiology, and toxicology. All taxonomic groups are covered from microbes, fungi, plants and animals. In order to better serve the community, we also now strongly encourage submissions of papers making use of modern molecular and genetic methods (population and functional genomics, transcriptomics, proteomics, epigenetics, quantitative genetics, association and linkage mapping) to address important questions in any of these disciplines and in an applied evolutionary framework. Theoretical, empirical, synthesis or perspective papers are welcome.
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