Boron confers salt tolerance through facilitating BnaA2.HKT1-mediated root xylem Na+ unloading in rapeseed (Brassica napus L.).

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-10-25 DOI:10.1111/tpj.17052
Yingpeng Hua, Minnan Pei, Haili Song, Ying Liu, Ting Zhou, Hongbo Chao, Caipeng Yue, Jinyong Huang, Guangyong Qin, Yingna Feng
{"title":"Boron confers salt tolerance through facilitating BnaA2.HKT1-mediated root xylem Na<sup>+</sup> unloading in rapeseed (Brassica napus L.).","authors":"Yingpeng Hua, Minnan Pei, Haili Song, Ying Liu, Ting Zhou, Hongbo Chao, Caipeng Yue, Jinyong Huang, Guangyong Qin, Yingna Feng","doi":"10.1111/tpj.17052","DOIUrl":null,"url":null,"abstract":"<p><p>Boron (B) is an important limiting factor for plant growth and yield in saline soils, but the underlying molecular mechanisms remain poorly understood. In this study, we found that appropriate B supply obviously complemented rapeseed (Brassica napus L.) growth under salinity accompanied by higher biomass production and less reactive oxygen species accumulation. Determination of Na<sup>+</sup> content in shoots and roots indicated that B significantly repressed root-to-shoot Na<sup>+</sup> translocation, and non-invasive micro-tests of root xylem sap demonstrated that B increased xylem Na<sup>+</sup> unloading in the roots of rapeseed plants under salinity. Comparative transcriptomic profiling revealed that B strongly upregulated BnaHKT1s expression, especially BnaA2.HKT1, in rapeseed roots exposed to salinity. In situ hybridizations analysis showed that BnaA2.HKT1 was significantly induced in root stelar tissues by high B (HB) under salinity. Green fluorescent protein and yeast heterologous expression showed that BnaA2.HKT1 functioned as a plasma membrane-localized Na<sup>+</sup> transporter. Knockout of BnaA2.HKT1 by CRISPR/Cas9 resulted in hypersensitive of rapeseed plants to salinity even under HB condition, with higher shoot Na<sup>+</sup> accumulation and lower biomass production. By contrast, overexpression of BnaA2.HKT1 ameliorated salinity-induced growth inhibition under B deficiency and salinity. Overall, our results proposed that B functioned as a positive regulator for the rapeseed growth and seed production under salt stress through facilitating BnaA2.HKT1-mediated root xylem Na<sup>+</sup> unloading. This study may also provide an alternative strategy for the improvement of crop growth and development in saline soils.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":null,"pages":null},"PeriodicalIF":6.2000,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://doi.org/10.1111/tpj.17052","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Boron (B) is an important limiting factor for plant growth and yield in saline soils, but the underlying molecular mechanisms remain poorly understood. In this study, we found that appropriate B supply obviously complemented rapeseed (Brassica napus L.) growth under salinity accompanied by higher biomass production and less reactive oxygen species accumulation. Determination of Na+ content in shoots and roots indicated that B significantly repressed root-to-shoot Na+ translocation, and non-invasive micro-tests of root xylem sap demonstrated that B increased xylem Na+ unloading in the roots of rapeseed plants under salinity. Comparative transcriptomic profiling revealed that B strongly upregulated BnaHKT1s expression, especially BnaA2.HKT1, in rapeseed roots exposed to salinity. In situ hybridizations analysis showed that BnaA2.HKT1 was significantly induced in root stelar tissues by high B (HB) under salinity. Green fluorescent protein and yeast heterologous expression showed that BnaA2.HKT1 functioned as a plasma membrane-localized Na+ transporter. Knockout of BnaA2.HKT1 by CRISPR/Cas9 resulted in hypersensitive of rapeseed plants to salinity even under HB condition, with higher shoot Na+ accumulation and lower biomass production. By contrast, overexpression of BnaA2.HKT1 ameliorated salinity-induced growth inhibition under B deficiency and salinity. Overall, our results proposed that B functioned as a positive regulator for the rapeseed growth and seed production under salt stress through facilitating BnaA2.HKT1-mediated root xylem Na+ unloading. This study may also provide an alternative strategy for the improvement of crop growth and development in saline soils.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
硼通过促进油菜籽(Brassica napus L.)根木质部 Na+ 卸载来赋予其耐盐性。
硼(B)是盐碱土壤中植物生长和产量的一个重要限制因素,但其潜在的分子机制仍然鲜为人知。在这项研究中,我们发现适当的硼供应明显促进了油菜(Brassica napus L.)在盐度条件下的生长,同时提高了生物量产量,减少了活性氧积累。芽和根中 Na+ 含量的测定表明,硼能显著抑制根到芽的 Na+ 转化,根木质部汁液的非侵入性微量测试表明,硼能增加盐度条件下油菜根木质部 Na+ 的卸载。转录组比较分析表明,硼能强烈上调盐渍化油菜根中 BnaHKT1s 的表达,尤其是 BnaA2.HKT1。原位杂交分析表明,在盐度条件下,高B(HB)显著诱导根茎组织中BnaA2.HKT1的表达。绿色荧光蛋白和酵母异源表达表明,BnaA2.HKT1具有质膜定位Na+转运体的功能。通过 CRISPR/Cas9 基因敲除 BnaA2.HKT1,即使在 HB 条件下,油菜植株对盐度也不敏感,芽的 Na+ 积累较高,生物量产量较低。相比之下,过表达 BnaA2.HKT1 可改善 B 缺乏和盐度条件下盐度诱导的生长抑制。总之,我们的研究结果表明,在盐胁迫下,B通过促进BnaA2.HKT1介导的根木质部Na+卸载,对油菜籽的生长和种子产量起着积极的调节作用。这项研究也可为改善盐碱地作物的生长和发育提供另一种策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
期刊最新文献
OsbHLH6, a basic helix-loop-helix transcription factor, confers arsenic tolerance and root-to-shoot translocation in rice. Photosystem rearrangements, photosynthetic efficiency, and plant growth in far red-enriched light. Tomato MADS-RIN regulates GAME5 expression to promote non-bitter glycoalkaloid biosynthesis in fruit. RETRACTION: Genotypic and phenotypic characterization of a large, diverse population of maize near-isogenic lines. TaWRKY24 integrates the tryptophan metabolism pathways to participate in defense against Fusarium crown rot in wheat.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1