Salivary Cortisol Detection with a Fully Inkjet-Printed Paper-Based Electrochemical Sensor.

IF 3 3区工程技术 Q2 CHEMISTRY, ANALYTICAL Micromachines Pub Date : 2024-10-12 DOI:10.3390/mi15101252

Miguel Zea, Hamdi Ben Halima, Rosa Villa, Imad Abrao Nemeir, Nadia Zine, Abdelhamid Errachid, Gemma Gabriel

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Abstract

Electrochemical paper-based analytical devices (ePADs) offer an innovative, low-cost, and environmentally friendly approach for real-time diagnostics. In this study, we developed a functional all-inkjet paper-based electrochemical immunosensor using gold (Au) printed ink to detect salivary cortisol. Covalent binding of the cortisol monoclonal antibody onto the printed Au surface was achieved through electrodeposition of 4-carboxymethylaniline (CMA), with ethanolamine passivation to prevent non-specific binding. The ePAD exhibited a linear response within the physiological cortisol range (5-20 ng/mL), with sensitivities of 25, 23, and 19 Ω·ng/mL and R² values of 0.995, 0.979, and 0.99, respectively. Additionally, interference studies against tumor necrosis factor-α (TNF-α) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) yielded excellent results. This novel ePAD, fabricated using inkjet printing technology on paper, simplifies the process, reduces environmental impact, and lowers fabrication costs.

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用全喷墨打印纸电化学传感器检测唾液皮质醇

基于电化学纸的分析装置（ePAD）为实时诊断提供了一种创新、低成本和环保的方法。在这项研究中，我们利用金（Au）印刷油墨开发了一种功能性全喷墨纸基电化学免疫传感器，用于检测唾液皮质醇。通过电沉积 4-羧甲基苯胺（CMA），将皮质醇单克隆抗体共价结合到印刷金表面，并用乙醇胺钝化以防止非特异性结合。ePAD 在生理皮质醇范围（5-20 ng/mL）内表现出线性响应，灵敏度分别为 25、23 和 19 Ω-ng/mL，R2 值分别为 0.995、0.979 和 0.99。此外，对肿瘤坏死因子-α（TNF-α）和 N 端前 B 型钠尿肽（NT-proBNP）的干扰研究也取得了优异的结果。这种新型 ePAD 采用纸张喷墨打印技术制造，简化了工艺流程，减少了对环境的影响，降低了制造成本。

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来源期刊

Micromachines NANOSCIENCE & NANOTECHNOLOGY-INSTRUMENTS & INSTRUMENTATION

CiteScore

5.20

自引率

14.70%

发文量

1862

审稿时长

16.31 days

期刊介绍： Micromachines (ISSN 2072-666X) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to micro-scaled machines and micromachinery. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.