Antimicrobial susceptibility of Clostridium botulinum group III field strains isolated in Europe from animal outbreaks

Abstract

Neurotoxins produced by Clostridium (C.) botulinum group III are responsible for the majority of botulism outbreaks occurring in animals and in this study we report the drug susceptibility of 71 field strains. The minimum inhibitory concentration (MIC) of 13 antimicrobials was established through the agar dilution method. The MIC₅₀ matched or differed for one or two dilutions from MIC₉₀ of the same antimicrobial, showing a unimodal distribution of the MIC values, irrespective of the geographical origin, the animal source and the toxinotype of the strain. Beta-lactams and rifampin showed the lowest MIC values, while gentamicin, polymyxin B and sulfamethoxazole showed the highest MICs. As for similar studies conducted in human botulism, the results could be helpful to avoid the administration of antimicrobials that could worsen the health condition of the affected animals and to develop selective media for the isolation of these fastidious anaerobes. Indeed, the isolation of the strain from affected animals and from environmental samples is important to perform epidemiological studies based on the genetic characterization and to produce tailor-made vaccines.