VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture.

IF 10.6 Q1 HORTICULTURE Molecular Horticulture Pub Date : 2024-10-25 DOI:10.1186/s43897-024-00117-z
Yan Xu, Zhengxin Lv, Muhammad Aamir Manzoor, Linhong Song, Maosen Wang, Lei Wang, Shiping Wang, Caixi Zhang, Songtao Jiu
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Abstract

The D14 protein, an alpha/beta hydrolase, is a key receptor in the strigolactone (SL) signaling pathway. However, the response of VvD14 to SL signals and its role in grapevine root architecture formation remain unclear. This study demonstrated that VvD14c was highly expressed in grapevine tissues and fruit stages than other VvD14 isoforms. Application of GR24, an SL analog, enhanced the elongation and diameter of adventitious roots but inhibited the elongation and density of lateral roots (LRs) and increased VvD14c expression. Additionally, GR24 is nested within the VvD14c pocket and strongly bound to the VvD14c protein, with an affinity of 5.65 × 10-9 M. Furthermore, VvD14c interacted with grapevine MORE AXILLARY GROWTH 2 (VvMAX2) in a GR24-dependent manner. Overexpression of VvD14c in the d14 mutant and VvMAX2 in the max2 Arabidopsis mutant reversed the increased LR number and density, as well as primary root elongation. Conversely, homologous overexpression of VvD14c and VvMAX2 resulted in reduced LR number and density in grapevines. VvMAX2 directly interacted with LATERAL ORGAN BOUNDARY (VvLOB) and VvLBD19, thereby positively regulating LR density. These findings highlight the role of SLs in regulating grapevine root architecture, potentially via the VvD14c-VvMAX2-VvLOB/VvLBD19 module, providing new insights into the regulation of root growth and development in grapevines.

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VvD14c-VvMAX2-VvLOB/VvLBD19模块参与了绞股蓝内酯介导的葡萄根系结构调控。
D14蛋白是一种α/β水解酶,是绞股蓝内酯(SL)信号通路中的一个关键受体。然而,VvD14对SL信号的反应及其在葡萄根系结构形成中的作用仍不清楚。本研究表明,与其他 VvD14 同工型相比,VvD14c 在葡萄组织和果实阶段的表达量较高。施用 SL 类似物 GR24 可增强不定根的伸长和直径,但会抑制侧根(LRs)的伸长和密度,并增加 VvD14c 的表达。此外,GR24 嵌套在 VvD14c 口袋中,与 VvD14c 蛋白结合力很强,亲和力为 5.65 × 10-9 M。此外,VvD14c 与葡萄 MORE AXILLARY GROWTH 2(VvMAX2)以一种依赖 GR24 的方式相互作用。在拟南芥 d14 突变体中过表达 VvD14c 和在 max2 突变体中过表达 VvMAX2 可逆转 LR 数量和密度的增加以及主根的伸长。相反,同源过表达 VvD14c 和 VvMAX2 会导致葡萄树的 LR 数量和密度减少。VvMAX2 直接与 LATERAL ORGAN BOUNDARY (VvLOB) 和 VvLBD19 相互作用,从而对 LR 密度起到正向调节作用。这些发现强调了SLs在调节葡萄根系结构中的作用,可能是通过VvD14c-VvMAX2-VvLOB/VvLBD19模块,为葡萄根系生长和发育的调节提供了新的见解。
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来源期刊
Molecular Horticulture
Molecular Horticulture horticultural research-
CiteScore
8.00
自引率
0.00%
发文量
24
审稿时长
12 weeks
期刊介绍: Aims Molecular Horticulture aims to publish research and review articles that significantly advance our knowledge in understanding how the horticultural crops or their parts operate mechanistically. Articles should have profound impacts not only in terms of high citation number or the like, but more importantly on the direction of the horticultural research field. Scope Molecular Horticulture publishes original Research Articles, Letters, and Reviews on novel discoveries on the following, but not limited to, aspects of horticultural plants (including medicinal plants): ▪ Developmental and evolutionary biology ▪ Physiology, biochemistry and cell biology ▪ Plant-microbe and plant-environment interactions ▪ Genetics and epigenetics ▪ Molecular breeding and biotechnology ▪ Secondary metabolism and synthetic biology ▪ Multi-omics dealing with data sets of genome, transcriptome, proteome, metabolome, epigenome and/or microbiome. The journal also welcomes research articles using model plants that reveal mechanisms and/or principles readily applicable to horticultural plants, translational research articles involving application of basic knowledge (including those of model plants) to the horticultural crops, novel Methods and Resources of broad interest. In addition, the journal publishes Editorial, News and View, and Commentary and Perspective on current, significant events and topics in global horticultural fields with international interests.
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